The possible occurrence of phosphorylation of proadrenocorticotropin(ACTH)/endorphin-derived peptides in the rat was examined by incubating dissociated anterior and intermediate pituitary cells with a %amino acid plus 32Pi. In both lobes of the pituitary newly synthesized pro-ACTH/endorphin was labeled with 32P. In the intermediate pituitary, corticotropin-like intermediate lobe peptide (CLIP) ...

BACKGROUND Preterm neonates undergo many painful procedures as part of their standard care in the neonatal intensive care unit. However, pain treatment is inadequate in many of these routine procedures. In the present study, we investigated the impact and mechanism of combined music and touch intervention (CMT) on the pain response in premature infants. METHODS Sixty-two preterm neonates (ges...

The repeated intermittent administration of amphetamine (AMP) produces an enduring enhancement in the response of dopamine (DA) systems in the brain to a subsequent "challenge" with amphetamine. However, former amphetamine addicts are not only hypersensitive to amphetamine, but also to "physical or psychological stress". This suggests that sensitization to amphetamine may change the response of...

ABSTRAK Nyeri persalinan merupakan pengalaman yang menyakitkan bagi beberapa Wanita disebabkan fisiologi dan psikologis. Ketakutan akan nyeri adalah salah satu alasan paling penting membuat wanita melakukan operasi caesar. berlebihan pada ibu bersalin dapat menyebabkan keinginan untuk segera mengakhiri masa persalinan. Persalinan fenomena alami namun rasa sakit menyertai sering dianggap parah b...

The possible involvement of 0-endorphin in the antihypertensive action of clonidine and a-methyldopa, drugs acting on central a2-adrenergic receptors, has been examined in rats with spontaneous, steroid-salt, or one-kidney, one-clip renal hypertension. In all three groups of rats, the hypotensive effect of a single intraperitoneal injection of 100 mg/kg of a-methyldopa was partially reversed by...

Τhe aim of this study was to examine the effects of the consumption of foods of various glycemic index values on performance, β-endorphin levels and substrate (fat and carbohydrate) utilization during prolonged exercise. Eight untrained healthy males underwent, in a randomized counterbalanced design, three experimental conditions under which they received carbohydrates (1.5 gr. kg-1 of body wei...

An expression vector was constructed that placed the cDNA for human neuropeptide Y (NPY) under the control of the mouse metallothionein promoter and was used to transfect the AtT-20 mouse anterior pituitary corticotrope cell line. AtT-20 cells normally process the pro-ACTH/endorphin precursor but do not produce detectable levels of NPY. The resulting AtT-20/NPY cell line (Mt.NPY1a) was used to ...

BACKGROUND Reports of reduced pain sensitivity in autism have prompted opioid theories of autism and have practical care ramifications. Our objective was to examine behavioral and physiological pain responses, plasma beta-endorphin levels and their relationship in a large group of individuals with autism. METHODOLOGY/PRINCIPAL FINDINGS The study was conducted on 73 children and adolescents wi...

AIM The present study was undertaken with the objective to obtain insight into the dynamics of the release of β-endorphin, adrenocorticotrophic hormone (ACTH) and cortisol in response to machine milking in dairy cows. MATERIALS AND METHODS A total of 10 healthy multiparous lactating Italian Friesian dairy cows were used in the study. Animals were at the 4(th)-5(th) month of pregnancy and were...

Prolactin plays major roles in maintaining the corpora lutea of pregnancy and in the synthesis of milk during lactation. The hypothalamic mechanisms involved in these functions have been investigated. Mating leads to a surge of prolactin and programs daily surges during early pregnancy. The expression of Fos-immunoreactivity shows that mating activates several hypothalamic nuclei, particularly ...