Repetitive-element PCR (rep-PCR) with primers based on repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) repeated DNA sequences was used for genomic finger-printing of Bartonella species. This technique was applied by using either extracted genomic DNA or preparations of whole bacterial cells directly. PCR fingerprints with either the REP-based p...

In this study, the suitability of two repetitive-element-based PCR (rep-PCR) assays, enterobacterial repetitive intergenic consensus (ERIC)-PCR and BOX-PCR, to rapidly characterize Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis (CF) was examined. ERIC-PCR utilizes paired sequence-specific primers and BOX-PCR a single primer that target highly conserved repetitive ele...

shigella sonnei is considered as a major cause of diarrheal disease in both developing and developed countries. iran is one of the endemic areas of shigellosis. the present study was undertaken to investigate the antibiotic susceptibility and genetic relatedness of s. sonnei strains isolated from pediatric patients in tehran, iran.the study included all s. sonnei strains isolated from pediatric...

shigellosis is one of the important gastrointestinal bacterial infections, particularly among children of developing countries such as iran. antibiotic susceptibility pattern and genetic typing for epidemiological purposes are of significant issues in shigella infectious control. the aim of this study was to investigate the antibiotic susceptibility and genetic relationship among shigella strai...

Most species of the diazotrophic Proteobacteria Azoarcus spp. occur in association with grass roots, while A. tolulyticus and A. evansii are soil bacteria not associated with a plant host. To facilitate species identification and strain comparison, we developed a protocol for PCR-generated genomic fingerprints, using an automated sequencer for fragment analysis. Commonly used primers targeted t...

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...

background aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). objectives this study aimed to evaluate the frequency of aseptic meningitis caused by herpesviruses, namely herpes simplex types i and ii (hsv-1, hsv-2), epstein-barr virus (ebv), cytomegalovirus (...