Alginate lyase mainly produces active alginate oligosaccharides (AOS) by degrading via β-elimination process. In this study, the Pseudoalteromonas sp. Alg6B lyase-encoding gene alg6B-7 from polysaccharide (PL)-7 family was successfully cloned, sequenced, expressed in Escherichia coli. Based on rational design and amino acid sequence alignment of various sources, four positive mutants were obtai...

Pesticides are indispensable in today’s agriculture. Methyl-parathion hydrolase (MPH, E.C.3.1.8.1) could hydrolyze organophosphorus pesticides, including methyl-parathion. MPH rehabilitate soil and water resources contaminated by pesticides. However, natural MPHs generally exhibited a low tolerance to high temperatures catalytic efficiency. In this study, we improved the efficiency toward methy...

The objective of this study was to develop a novel bacterially-triggered micro-particular system of de-esterified tragacanth (DET) in combination with Eudragit S-100 coated capsules for colon drug delivery of 5-fluorouracil (5-FU) using microemulsion method. The loading study was conducted at different drug-to-polymer ratios and cross-linker concentrations. The maximum loading efficiency was ac...

The objective of this study was to develop a novel bacterially-triggered micro-particular system of de-esterified tragacanth (DET) in combination with Eudragit S-100 coated capsules for colon drug delivery of 5-fluorouracil (5-FU) using microemulsion method. The loading study was conducted at different drug-to-polymer ratios and cross-linker concentrations. The maximum loading efficiency was ac...

Lignin peroxidase can degrade lignin in a relatively high efficiency, the highly efficient application of for environmental prevention and governance was promoted by exploring effects small molecule substances on activities. In this study, (LiP) gene from Irpex Lacteus heterologous expressed Escherichia coli. The activity recombinant LiP 11.2 U/mg, optimum temperature 37℃, pH 3.5. range 20-45℃ ...

Abstract Residues of antibiotics in water resources and wastewater have been significant environmental public health problems. The current study developed a high-efficiency enzymatic nanobiocatalyst for the degradation beta-lactam antibiotics. For this purpose, metallo-beta-lactamase IMP-1 was obtained by cloned blaIMP gene overexpressed Escherichia coli. 2.6 mg purified enzyme used immobilizat...

sugar beet pulp is one of the sugar industries by-products which can be used for cellulase enzyme production, enzymatic saccharification, and alcohol production due to its high percentage of lingo-cellulosic content. trichoderma spp. is an important fungus that produces a wide range of cellulytic enzymes. in this study, cellulase enzyme was produced by placing sugar beet pulp in trichoderma fer...

Hydrocarbon synthesis from (waste)oils enabled by a cascade of lipase-catalysed hydrolysis and decarboxylase-catalysed decarboxylation has become an active area research en route to alternative, biobased fuels. However, Poor substrate transport efficiency is major issue causing low reaction rates. This study focused on protein self-assembly strategy based SpyTag/SpyCatcher overcome diffusion li...

Enzyme engineering via immobilization techniques is a suitable approach for improving enzyme function and stability and is superior to the other chemical or biological methods. In this study chitosan nanoparticles were synthesized using the Ionic Gelation method and were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Transmission Electron...

Eversa® Transform 2.0 lipase (ET2) is a recent formulation derived from the Thermomyces lanuginosus cultivated on Aspergillus oryzae and specially designed for biodiesel production. Since it has not been available long time, research efficiency of this enzyme in other applications remains unexplored. Moreover, even though launched as free enzyme, its immobilization may extend scope ET2 applicat...