the engineering of transgenic canola (brassica napus l. ) to make tolerance to the broad-spectrum herbicide, glyphosate, is one of the most effective approaches for weed management. glyphosate inhibits the enzyme epsps (5-enolpyruvylshikimate-3-phosphate synthase) enzyme which functions in the shikimate pathway and has a key role in biosynthesis of aromatic amino acids required for survival of ...

Three enzymes that cause inhibition of mRNA translation, eukaryotic initiation factor 2 protein kinase PK-i, oligoisoadenylate synthetase E, and phosphodiesterase 2'-PDi, have been recently isolated from interferon-treated cells. We show that the rise in these three enzyme activities may be used to study the response of uninfected cells to interferon. For each enzyme, a specific microassay that...

a wide range of biological activities of garlic in vitro and in vivo have been verified including its antioxidant, antitumor and anti-inflammatory effects. indoleamine 2,3-dioxygenase (ido) is an enzyme widely distributed in mammals and is inducible preferentially by ifn-γ. ido degrades the essential amino acid tryptophan to form n-formyl kynurenine. in the present in vitro study, the modulator...

1. Folate deficiency is commonly found in alcoholic subjects although the causative mechanism is uncertain. It has been suggested that microsomal enzyme induction resulting from chronic alcohol ingestion might accelerate the rate of folate catabolism thus causing deficiency. 2. By using an experimental animal model to determine the rate of catabolism of [3H]pteroylglutamate (folic acid) by the ...

Induction of the endogenous human NAD(P)H:quinone oxidoreductase (HQOR1) gene in the human hepatoma cell line HepG2 was measured at both the enzyme activity and RNA levels after exposure to a variety of industrial compounds. An RNA probe was designed that was complementary to portions of both the coding region and the 3'-nontranslated region unique to the largest (2.7-kilobase) HQOR1 transcript...

The engineering of transgenic canola (Brassica napus L. ) to make tolerance to the broad-spectrum herbicide, glyphosate, is one of the most effective approaches for weed management. Glyphosate inhibits the enzyme EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) enzyme which functions in the shikimate pathway and has a key role in biosynthesis of aromatic amino acids required for survival of ...

1. An injection of D-galactosamine at the same time as an injection of dexamethasone inhibits the increase of rat liver tyrosine aminotransferase activity normally observed following injection of dexamethasone alone. The galactosaminemediated inhibition is dose dependent: a 50% inhibition of the increase of enzyme activity is observed at 10 mg of galactosamine .HCl per 100 g of body weight, and...

1. An injection of D-galactosamine at the same time as an injection of dexamethasone inhibits the increase of rat liver tyrosine aminotransferase activity normally observed following injection of dexamethasone alone. The galactosaminemediated inhibition is dose dependent: a 50% inhibition of the increase of enzyme activity is observed at 10 mg of galactosamine .HCl per 100 g of body weight, and...

Zixor induction occurred only with an increase in the amount of free T3. Free T4 levels even tended to decline. At same time, trend was observed TTG. Our research shows that there is a relationship between functional status liver monooxygenase system and thyroid hormones body. However, question degree closeness this connection, is, whether connection direct or indirect, remains open. So far, st...