The bacterial Sox (sulfur oxidation) pathway is an important route for the oxidation of inorganic sulfur compounds. Intermediates in the Sox pathway are covalently attached to the heterodimeric carrier protein SoxYZ through conjugation to a cysteine on a protein swinging arm. We have investigated how the carrier protein shuttles intermediates between the enzymes of the Sox pathway using the int...

The mechanism of protein kinase CK2 (CK2) activity stimulation by polylysine has been studied by surface plasmon resonance (SPR). The kinetics of the polylysine interaction with a peptide substrate of the enzyme, and with the enzyme itself, have been investigated. A peptide containing a threonine (T) residue surrounded by a cluster of negatively charged acidic [arginine (R) and glutamic acid (E...

In most ecosystems, the degradation of complex organic material depends on extracellular enzymes produced by microbes. These enzymes can exist in bound or free form within the soil, but the dynamics of these different enzyme pools remain uncertain. To address this uncertainty, I determined rates of enzyme turnover in a volcanic soil with and without added enzymes. I also tested whether or not s...

Abstract This chapter attempts to provide an account of works that have attempted to characterize protein–protein interaction interfaces, with fractal dimension. However, such characterization of interfaces is not solely dependent upon interface roughness. Without involving the biophysical factors, we will concentrate only on geometric characterization of these interfaces. To be specific, we wi...

The interactions of Mn2+, nucleotides, monosaccharide substrates, and the modifier protein alpha-lactalbumin with galactosyltransferase have been studied by difference spectroscopy. MnCl2, required for significant binding of UDP-galactose or UDP to the enzyme, does not exhibit specific interactions with tyrosine or tryptophan residues. The interaction of UDP-galactose or UDP with galactosyltran...

Abstract This chapter attempts to provide an account of works that have attempted to characterize protein–protein interaction interfaces, with fractal dimension. However, such characterization of interfaces is not solely dependent upon interface roughness. Without involving the biophysical factors, we will concentrate only on geometric characterization of these interfaces. To be specific, we wi...

The appearance of brown spots and wrinkles can be a sign aging which increase the risk cancer. Compounds in broadleaf mahogany seeds (Swietenia macrophylla) contains many compounds is known to have anticancer activity. purpose this study was determine these plants potential antiaging by analyzing their molecular docking interaction on MMP-1 enzyme (matrix metalloproteinase), NEP (neprilysin), P...

Alzheimer's disease is the most common progressive neurodegenerative disorder that effects large population of society especially elderly people. Environmental and/or genetic factors contribute to become a pivotal health problem but this relationship remains ambiguous. Globally growing prevalence Alzheimer’s requires understand cellular pathways lead and develop new strategies for prevention tr...

This research presents a synthesis and characterization of new 6,7-dichloro-5,8-quinolinedione derivatives with various groups at the C2 position. Chemical structures were examined by spectroscopic methods. The quantum chemical parameters calculated using DFT method showed that these are highly reactive towards nucleophilic target. molecular electrostatic potential map (MEP) regions localized n...

The ocr protein, the product of gene 0.3 of bacteriophage T7, is a structural mimic of the phosphate backbone of B-form DNA. In total it mimics 22 phosphate groups over approximately 24 bp of DNA. This mimicry allows it to block DNA binding by type I DNA restriction enzymes and to inhibit these enzymes. We have determined that multiple ocr dimers can bind stoichiometrically to the archetypal ty...