Glycerolipid synthesis from [3H]glycerol and [l*C]dihydroxyacetone operated in enqmically isolated rat hepatocytes at a rate similar to that in the intact organ. Addition of albumin-bound fatty acids with more than 12 carbon atoms to the incubation medium markedly stimulated triacylglycerol synthesis from [3H]glycerol whereas the effects on phospholipid synthesis were smaller and more dependent...

Fatty acids of winged bean seed oil were examined by gas chromatography-mass spectrometry of their pyrrolidide derivatives. Oleic and linoleic acids were determined to be the major unsaturated fatty acids in the oil. Long chain saturated fatty acids, such as behenic and lignoceric acids, were found in relatively high amounts as compared with other edible seed oils. The fatty acids that could no...

Effect of Tower rapeseed oil (RO) containing lower (0.4%) erucic acid on the piglet liver was studied by electron microscopy. The animals were placed in two groups of four and were fed the diets for eight weeks. Animals in the treated group were given a basal diet comprising corn-soybean plus Tower RO at a 10% concentration; animals that were given the basal diet with no added oil served as the...

We examined the ability of erucic acid (22:1n-9) to cross the blood-brain barrier (BBB) by infusing [14-14C]22:1n-9 (170 microCi/kg, iv and icv) into awake, male rats. [1-14C]arachidonic acid (20:4n-6) [intravenous (i.v.)] was the positive control. After i.v. infusion, 0.011% of the plasma [14-14C]22:1n-9 was extracted by the brain, compared with 0.055% of the plasma [1-14C]20:4n-6. The [14-14C...

Human or rat microsomal 5 alpha-reductase activity, as measured by enzymic conversion of testosterone into 5 alpha-dihydrotestosterone or by binding of a competitive inhibitor, [3H]17 beta-NN-diethulcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one ([3H]4-MA) to the reductase, is inhibited by low concentrations (less than 10 microM) of certain polyunsaturated fatty acids. The relative inhibitory...