Bioassay was conducted to determine the susceptibility level of the adults of Callosobruchus chinensis, Sitophilus granarius and Tribolium casteneum against four BT isolates (1i, 27s, 27f and HD73) where mortality effects were counted from 24 to 192 hours (8 days) at 24 hours of intervals. All strains of BT were highly active against C. chinensis and the LC50 were found to be 0.30, 0.73, 0.62 a...

AntiHunter is a new web-based tool for the identification of expressed sequence tag (EST) antisense transcripts from BLAST output. In order to perform an analysis, user is required to input a genomic sequence plus an associated list of transcript names and coordinates of the genomic region (i.e. genome annotation). After masking the repeated regions (if any), program will perform a BLASTN searc...

Résumé L’analyse d’accessibilité est généralement l’approche utilisée pour la validation des protocoles. Le problème posé par l’application de cette technique est celui de l’explosion combinatoire. Dans cet article, nous proposons une nouvelle technique de validation de protocoles appelée Reverse Leaping Reachability Analysis (RLRA). Au lieu de parcourir tout l’espace des états de protocole, no...

Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....

Pneumoperitoneum may give rise to several respiratory changes; nevertheless, no comprehensive analysis of respiratory mechanics has been performed under this condition. Respiratory mechanics and thoracoabdominal morphometry were evaluated in six sedated, anaesthetized, paralysed, and mechanically-ventilated rats before (control) and during pneumoperitoneum. After airway occlusion at end-inspira...

Inspite of the large body of genomic data obtained from the transcriptome of Zingiber officinale, very few studies have focused on the identification and characterization of miRNAs in gingerol biosynthesis. Zingiber officinale transcriptome was analyzed using EST dataset (38169 total) deposited in public domains. In this paper computational functional annotation of the available ESTs and identi...

Comparative population genetic analyses of traditional and emergent molecular markers aid in determining appropriate use of new technologies. The bocaccio rockfish Sebastes paucispinis is a high gene-flow marine species off the west coast of North America that experienced strong population decline over the past 3 decades. We used 18 anonymous and 13 gene-associated simple sequence repeat (SSR) ...