نتایج جستجو برای: exonucleases
تعداد نتایج: 480 فیلتر نتایج به سال:
Mitochondrial RNAs in Trypanosoma brucei undergo posttranscriptional RNA editing and polyadenylation. We previously showed that polyadenylation stimulates turnover of unedited RNAs. Here, we investigated the role of polyadenylation in decay of edited RPS12 RNA. In in vitro turnover assays, nonadenylated fully edited RNA degrades significantly faster than its unedited counterpart. Rapid turnover...
The regulation of gene expression can take place at many stages subsequent to transcriptional activation. In eukaryotic systems the messenger RNA (mRNA) is subject to processing, nucleocytoplasmic transport, translation and degradation. Each of these points serves as a checkpoint at which the ultimate production of encoded protein can be regulated. Studies in the past two decades have shown tha...
Inactive forms of endo-exonuclease, activated in vitro by treatment with trypsin, have been identified in human leukaemic CEM and MOLT-4 cells. They comprise over 95% of the total single-strand DNase activity in nuclei and are mainly bound to chromatin and the nuclear matrix. The activated enzyme had Mg2+(Mn2+)-dependent, Ca(2+)-stimulated activities with single- and double-strand DNAs and RNA ...
The origins of the recombinant DNA technology can be traced back to the discovery of restriction enzymes and the generation of the first recombinant DNA molecule over 40 years ago (1–3). Since then, and with the emergence of PCR, scientists have generated a number of elegant cloning systems that enable the manipulation of DNA fragments in various ways (for recent reviews see Refs 4–7). Some rem...
Mismatch Repair in Bacteria and Eukaryotes Mismatch repair in the bacterium Escherichia coli is initiated when a homodimer of MutS binds as an asymmetric clamp to DNA containing a variety of base-base and insertion-deletion mismatches. The MutL homodimer then couples MutS recognition to the signal that distinguishes between the template and nascent DNA strands. In E. coli, the lack of adenine m...
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