Creation of transgenic (knockout) mice deficient in calpain small (30 kDa) subunit gene was undertaken to clarify the proposed role of the small subunit for calpain proteolytic activity and to gain insight into the importance of the gene in the whole animal. The gene was targeted and disrupted in embryonic stem cells by homologous recombination, and chimeric mice were generated. Heterozygous F1...

Ocean warming is a threat to marine biodiversity, as it can push species beyond their physiological limits. Detrimental effects occur when poikilotherms are exposed conditions thermal optima. However, acclamatory mechanisms, such plasticity, may enable compensation of detrimental if experienced during development or across generations. Studies evaluating the molecular responses fishes have most...

The stability of transgene transmission, milk production and milk content of recombinant human protein C (rhPC) in transgenic rabbits of the F3 generation was determined. Transgenic rabbits carrying the 4.2kb mouse whey acid protein promoter and 9.4kb genomic human protein C were produced after mating transgenic (F2 generation) and non-transgenic rabbits. PCR analysis of F3 samples showed that ...

The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-generic hybridization in 2009 and 2010, 2 repeat, and 88 normal plants were obtained. The characteristics of leaves, stems and branches were the same as female for F1 generation, F2 ...

The study was conducted to investigate the ge-netic basis of kenaf fiber yield and quality In two sets of generations derived from 1X51 x Gha-na07 and Gregg x Ghana07 crosses. Analysis of generation means using weighted least squares procedure was performed on stalk dry weight, bast percentage, days to flowering, plant height, and basal stalk diameter . Analysis of generation means revealed tha...

(1) Let F , F1, F2 be finite sequences of elements of R and given r1, r2. If F1 = 〈r1〉 F or F1 = F a 〈r1〉 and if F2 = 〈r2〉 F or F2 = F a 〈r2〉, then ∑(F1−F2) = r1− r2. (2) Let F1, F2 be finite sequences of elements of R. If lenF1 = lenF2, then len(F1 +F2) = lenF1 and len(F1−F2) = lenF1 and ∑(F1 +F2) = ∑F1 +∑F2 and ∑(F1−F2) = ∑F1−∑F2. (3) Let F1, F2 be finite sequences of elements of R. If lenF1 ...

Associations between the parameters of resistance to coccidiosis and SNP in 3 candidate genes located on chromosome 1 [T cell receptor-beta (TCR-beta), myeloid leukemia factor 2 (MLF2), and lymphotactin] were determined. Single nucleotide polymorphisms were genotyped in 24 F1 generation and 290 F2 generation birds. Four SNP were identified in the lymphotactin gene, 12 were located in the TCR-be...