Exercise has been shown to increase the production of reactive oxygen species to a point that can exceed antioxidant defenses to cause oxidative stress. Dietary intake of antioxidants, physical activity levels, various antioxidants and oxidative stress markers were examined in 20 exercise-trained "athletes" and 20 age- and sex-matched sedentary "controls." Plasma F2-isoprostanes, antioxidant en...

The oxidation hypothesis of atherogenesis has been the focus of much research over the past 2 decades. However, randomized placebo-controlled trials evaluating the efficacy of vitamin E in preventing cardiovascular events in aggregate have failed to show a beneficial effect. Implicit in these trials is that the dose of vitamin E tested effectively suppressed oxidative stress status but this was...

OBJECTIVE We have previously reported evidence of an inverse association between a urinary F(2)-isoprostane and type 2 diabetes risk in a pilot case-control study nested within the Insulin Resistance Atherosclerosis Study (IRAS). Here, we report the results from the study extended to the entire IRAS cohort. RESEARCH DESIGN AND METHODS This prospective study included 138 incident type 2 diabet...

Calpain-3 is an intracellular cysteine protease, belonging to Calpain superfamily and predominantly expressed in skeletal muscle. In human melanoma cell lines and biopsies, we previously identified two novel splicing variants (hMp78 and hMp84) of Calpain-3 gene (CAPN3), which have a significant lower expression in vertical growth phase melanomas and, even lower, in metastases, compared to benig...

BACKGROUND AND PURPOSE Both platelet activation and lipid peroxidation are potential sources of vasoactive eicosanoids that can be produced via the cyclooxygenase pathway, ie, thromboxane (TX) A2, or by free radical-catalyzed peroxidation of arachidonic acid, ie, isoprostanes. We investigated the biosynthesis of TXA2 and F2-isoprostanes, as reflected by the urinary excretion of 11-dehydro-TXB2 ...

BACKGROUND An oxidative balance score (OBS) that combines pro- and antioxidant exposures was previously reported to be associated with incident sporadic colorectal adenoma. We extend the previous analyses by assessing associations of the OBS and colorectal adenoma with circulating biomarkers of oxidative stress [F2-isoprostanes (FIP) and fluorescent oxidation products (FOP)], and inflammation [...

Rett syndrome (RTT) is a pervasive neurodevelopmental disorder mainly linked to mutations in the gene encoding the methyl-CpG-binding protein 2 (MeCP2). Respiratory dysfunction, historically credited to brainstem immaturity, represents a major challenge in RTT. Our aim was to characterize the relationships between pulmonary gas exchange abnormality (GEA), upper airway obstruction, and redox sta...

F2-isoprostanes have been advocated as a specific and reliable marker of oxidative damage (1 ) despite a lack of data on their biological and analytical variation. Concurrent with a longitudinal study of oxidative stress during the menstrual cycle [the BioCycle Study (2 )], we have characterized the components of biological and analytical variation of free plasma F2isoprostanes in the context o...

Objective—To evaluate oxidative injury and inflammatory status in various rheumatic diseases by measuring the levels of isoprostanes and prostaglandins in serum and synovial fluid. Methods—The concentrations of 8-isoPGF2á (F2-isoprostane indicating oxidative injury) and 15-keto-dihydro-PGF2á (a major metabolite of prostaglandin F2á) were measured in both serum and synovial fluid aspirated from ...

Endothelial Dysfunction, Isoprostanes, and Copper Deficiency To the Editor: Following the tradition of using animal experiments to clarify and illustrate human pathology, Lavi et al1 sought similarities between people with ischemic heart disease and animals fed cholesterol. They detected a decreased effect of acetylcholine on coronary arteries in 8 of 20 angiogram patients with minimal coronary...