Histone H2AX promotes DNA double-strand break (DSB) repair and immunoglobulin heavy chain (IgH) class switch recombination (CSR) in B-lymphocytes. CSR requires activation-induced cytidine deaminase (AID) and involves joining of DSB intermediates by end joining. We find that AID-dependent IgH locus chromosome breaks occur at high frequency in primary H2AX-deficient B cells activated for CSR and ...

The induction of chromatid translocations by l-methyl-2-benzylhydrazine was studied in a number of malignant and normal mammalian cell types and in narcissus root cells. In three different mouse ascites tumors (Ehrlich, 2BF, and LI210) treated in vivo, translocations were induced. In the Ehrlich tumor, they were induced with unusually high frequency, involving, in some cells, a majority of the ...

ETEROZYGOUS autosomal translocations have been shown to affect recomHbination in the X chromosome in various ways (HINTON 1965); translocations with long interstitial distances increased recombination in X, those with short interstitial distances decreased recombination in X, and those with intermediate interstitial distances had little or no effect. Other incidental tests of translocation hete...

Two girls with congenital diaphragmatic herniae are reported. Both were discovered to have a balanced reciprocal translocation involving 8q22.3. In one girl the translocation was de novo, in the other it was maternally inherited. Uniparental disomy was excluded in both. 8q22.3 may be the location of a gene affecting development of ihe diaphragm. (J Med Genet 1994;31:735-737) Wessex Clinical Gen...

We have previously described a transcription unit on human chromosome 8, designated as PVT, that is consistently disrupted by the minority forms of translocations [t(2;8) and t(8;22)] in Burkitt's lymphoma. PVT begins 57 kilobase pairs downstream of the proto-oncogene MYC and is more than 200 kilobase pairs in length. In order to explore the pathogenic impact of translocations affecting PVT, we...

Translocation is one of the most frequently occurring human chromosomal aberrations. The constitutional t(11;22)(q23;q11), which is the only known recurrent non-Robertsonian translocation, represents a good model for studying translocations in humans. Here we demonstrate polymorphisms of the palindromic sequence at the t(11;22) breakpoint that affect the frequency of de novo translocations in s...

Recurrent chromosome translocations in nonhematological tumors are restricted to specific subtypes, and their mechanism is currently unknown. Analysis of the sequence data of 113 interchromosomal junctions derived from 77 Ewing's tumors carrying the characteristic t(11;22) translocation indicate that, in this tumor, translocations are initiated independently on each chromosome in regions that l...

Conservation Translocations edited by Martin J. Gaywood, John G. Ewen, Peter M. Hollingsworth and Axel Moehrenschlager (2022) 400 pp., Cambridge University Press, Cambridge, UK. ISBN 978-1-108-71457-0 (pbk), GBP 34.99. - Volume 57 Issue 4

Chromosomal region Ilq23 participates in a number of reciprocal translocations with specific regions of chromosomes 4, 9, 19, and others. These translocations are associated with acute lymphocytic leukemia and acute myelomonocytic, monocytic, and myelogenous leukemia. From a yeast artificial chromosome containing human DNA derived from Hq23 we cloned a DNA fragment which can be used as a probe ...