THE ROLE OF PROTEIN MEDIATED TRANSPORT IN REGULATING MITOCHONDRIAL LONG CHAIN FATTY ACID OXIDATION Graham Paul Holloway Advisor: University of Guelph, 2007 Professor L.L. Spriet This thesis is an investigation of the role of fatty acid translocase (FAT/CD36), plasma membrane associated fatty acid binding protein (FABPpm) and carnitine palmitoyltransferase I (CPTI) in transporting long chain fat...

We determined the effect of fat adaptation on metabolism and performance during 5 h of cycling in seven competitive athletes who consumed a standard carbohydrate (CHO) diet for 1 day and then either a high-CHO diet (11 g. kg(-1)x day(-1) CHO, 1 g x kg(-1) x day(-1) fat; HCHO) or an isoenergetic high-fat diet (2.6 g x kg(-1) x day(-1) CHO, 4.6 g x kg(-1) x day(-1) fat; fat-adapt) for 6 days. On ...

Energy expenditure and fat oxidation are both profoundly increased in exercise. In absolute terms, fat oxidation is greatest during exercise of moderate intensity (60-65 % maximum 0 2 uptake, vo2,,) when subjects following a mixed diet may oxidize between 0.3 and 0.7g fat/min, rFpresenting up to 60% of energy expended (Saltin & Astrand, 1993). Because the capacity of muscle fibres to synthesize...

Following a low carbohydrate diet, there is a shift towards more fat and less carbohydrate oxidation to provide energy to skeletal muscle, both at rest and during exercise. This review summarizes recent work on human skeletal muscle carbohydrate and fat metabolic adaptations to a low carbohydrate diet, focusing mainly on pyruvate dehydrogenase and pyruvate dehydrogenase kinase, and how these ch...

Background Green tea and green tea extract (GTE) consumption is associated with health, exercise performance, fat oxidation and weight loss owing to studies reporting positive effects accompanying chronic supplementation (>30 d). While the overall literature surrounding GTE is equivocal, little data exists examining the effects of acute supplementation prior to exercise (< 24 hr). The primary a...

The effect of fat feeding on adipocyte insulin binding was examined to expand a study of adaptive changes in plasma membrane functions. Cells from rats fed a high fat (L) diet for five to seven days bound less insulin and showed a decreased response to insulin (glucose oxidation) compared to those from rats fed a high glucose (G) diet. Both high and low affinity sites were influenced; the exten...

Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in v...

High-fat diets may promote growth, partly through their protein-sparing effects. However, high-fat diets often lead to excessive fat deposition, which may have a negative impact on fish such as poor growth and suppressive immune. Therefore, this study investigated the effects of a fat-rich diet on the mechanisms of fat deposition in the liver. Three-hundred blunt snout bream (Megalobrama amblyc...

BACKGROUND It takes several days to adapt to a high-fat diet. In an earlier study, we observed a large degree of interindividual variation in the capacity to adapt to a high-fat diet. We hypothesized that concurrent physical activity would accelerate fat oxidation during an isoenergetic high-fat diet. OBJECTIVE The objective of this study was to determine the effect of increased physical acti...