background: the advent of recombinant dna technology has facilitated heterologous expression of proteins from various sources in different host systems including escherichia coli. if a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Reverse transcriptase-mediated PCR has been used to isolate two distinct metallothionein (MT) cDNA species from RNA extracted from icefish liver, namely MT-I and MT-II. Northern blot analysis with these cDNA species revealed that significant endogenous levels of MT mRNA were present in liver tissues of normal animals despite the fact that no MT protein could be found accumulating in the same ti...

In November 2017, a group of farmed rainbow trout (Oncorhynchus mykiss) died in Yunnan Province, China, likely due to infection. These exhibited slow movement, no feeding, dark body color, exophthalmia, and occasional ulcers on the surface. Pathogens were isolated from diseased livers, head kidneys, spleens, eyes, strain was preliminarily identified as Acinetobacter johnsonii based morphology, ...

Mycobacteriosis is a chronic disease of freshwater and marine fish. In the present study, Mycobacterium fortuitum from a diseased gold fish, Carassius auratus was isolated and identified by biochemical and molecular (PCR) method. The isolate was found to be resistant to rifampicin, isoniazid, pyrazinamide and ethambutol and myoconda® a nanodrug combination of rifampicin, clarithromycin and clof...

A 50-day feeding trial was conducted to evaluate the effects of yeast nucleotide in diets on growth performance, serum immune indices and muscle composition of Ancherythroculter nigrocauda (mean initial body weight, 23.30±0.59 g). Seven isonitrogenous (approximately 42.76% crude protein) and isoenergetic (17.43 KJ g−1 gross energy) experimental diets with varying levels of yeast nucleotide (0[c...