The well-known methods for the determination of serum iron, which are based on colour reactions with bathophenanthroline disulfonate, Ferrozine® or Chromazurol B, require — even on a microscale — sample amounts between 0.1 and 1.0 nil of serum (1,2,3). These sample volumes are too large for paediatric purposes. Reduction of sample size is possible in flame atomic absorption spectrophotometry us...

We have established and evaluated a flameless graphite cuvette method for copper in serum. This atomic absorption method provides substantial improvement in sensitivity, adequate accuracy, and acceptable precision, and little sample preparation is required before the analysis. Standard addition studies and measurements of National Bureau of Standards materials indicated that the proposed meth...

a simple method has been developed for the preconcentration of lead (ii) based on the adsorption of its 1-(2-pyridyl azo)2-naphtol (pan) complex on aoctadecyl bonded silica cartridges. the influence of acidity, eluting agents, stability of the column, sample volume and interfering ions has been investigated in detail. the adsorbed complex could be eluted using environmentally benign hno34m and ...

We describe an inductively coupled plasma atomic emission spectrometer that has been adapted to perform routine, simultaneous, direct analyses of calcium, magnesium, copper, zinc, and iron in serum or urine without sample digestion or pretreatment. The system, constructed with inexpensive, readily available components, can analyze 1-mL or smaller samples. Results correlate nearly perfectly with...

A method for the preconcentration and determination of Cr(III), Co(II), Cu(II), Fe(III) and Pb(II) ions by atomic absorption spectrometry has been described. The method was based the collection of metal-calmagite complexes on a soluble cellulose nitrate membrane filter. The detection of the solution was obtained by flame atomic absorption spectrometry (FAAS) after completely dissolving the memb...

Total diet sample/Elements/Atomic absorption spectrometry/Element correlation Total diet samples were collected from two urban areas in Japan, Sapporo and Kyoto, during the period from 1967 to 1968. The samples, ashed by dry-ashing and acid-digestion, were analyzed for elements by atomic absorption spectroscopy with the flame and electrothermal atomization. Average daily intake found in the two...

The involvement of elements in the pathological process of primary hypertension has been established. The serum levels of copper, iron, zinc, sodium, potassium and calcium were studied by atomic absorption spectrophotometery and flame photometry in hypertensive and normal subjects. Serum copper and zinc levels were increased in hypertensive patients (25.5% and 14.7% respectively) as compar...

Pb and Cd in water samples were determined by cloud point extraction (CPE) with sodiumdiethyldithiocarbamate (DDTC) as the chelating agent and octylphenoxypolyethanl (Triton X-114) asthe surfactant prior to flame atomic absorption spectrometry (FAAS) determination. The main factorsof affecting the extraction and detection processes were optimized. The preconcentration of 10 mL ofstandard soluti...

Liquid chromatographs, coupled with graphite furnace atomic absorption spectrometers, have been widely used for the identification and quantification of trace element compounds. The quantification of the discontinuous signals from the spectrometer defining a chromatographic band is very much a matter of judgement and therefore prone to error. This paper describes a system which links a high-per...

A sensitivity comparison was made between conventional atomic absorption spectrophotometry (AAS) and conventional molecular absorption spectrophotometry (MAS) for the serum trace metals, copper, iron, and zinc. The sensitivity aspect considered was absorbances was obtained for concentrations in the solutions analyzed using unit lightpaths of 1 cm and 10 cm for MAS and AAS, respectively. A disti...