We present a highly sensitive capillary electrophoresis (CE) assay that combines transient, single-interface on-chip isotachophoresis (ITP) and a laser-induced confocal fluorescence detection setup. We performed experimental parametric studies to show the effects of microscope objective specifications and intensity of excitation laser on optimization of a high-sensitivity on-chip CE detection s...

We report interferometric observations of the classical galactic Cepheid ζ Gem with FLUOR (Fiber Linked Unit for Optical Recombination), installed at the IOTA (Infrared Optical Telescope Array) interferometer. Thanks to the high precision of the visibility measurements with FLUOR, it has been possible to estimate its mean uniform disk angular diameter to a relative precision of 5 % (θud = 1.98 ...

We demonstrate that the second-Stokes output from a diamond Raman laser, pumped by a femtosecond Ti:Sapphire laser, can be used to efficiently excite red-emitting dyes by two-photon excitation at 1,080 nm and beyond. We image HeLa cells expressing red fluorescent protein, as well as dyes such as Texas Red and Mitotracker Red. We demonstrate the potential for simultaneous two-color, two-photon i...

In order to discern aggregation in solution, we present a quantum mechanical analog of the photon statistics from fluorescent molecules diffusing through a focused beam. A generating functional is developed to fully describe the experimental physical system as well as the statistics. Histograms of the measured time delay between photon counts are fit by an analytical solution describing the sta...

The prevalence of caries among toddlers in Indonesia was about 81.5%. type that often occurs Early Childhood Caries (ECC). ECC a condition where there one or more tooth decay, the main cause consuming milk sugary drinks using bottle until fall asleep too which not balanced by oral hygiene maintenances. could be prevented treatment topical application fluor. One preventive agents for fluor Silve...

UNLABELLED Prospective experimental study in which we created a bony defect in the mastoids of rats and filled it up with hydroxyapatite to evaluate bone regeneration, to solve the problems of open cavities after mastoidectomies that frequently present with otorrhea, infection, granulation tissue and hearing loss. OBJECTIVE The aim was to evaluate bone regeneration in defects created in the m...

INTRODUCTION This study compares the outcome and complications of integrated hydroxyapatite implant and non-integrated orbital implants following enucleation in Asian patients. MATERIALS AND METHODS This is a retrospective study of enucleated patients with coralline hydroxyapatite implants versus non-integrated implants (acrylic, glass and silicone) at the Singapore National Eye Centre from J...

This study conducted plant growth tests using a rhizobox system to quantitatively determine the distance of immobilization lead migrating from contaminated soil into uncontaminated rhizosphere soil, and to assess the lead phases accumulated in rhizosphere soil by sequential extraction. Without the hydroxyapatite, exchangeable lead fractions increased as the rhizosphere soil got closer to the co...

The amount of fluoride adsorbed onto hydroxyapatite increased as the aqueous fluoride concentration increased. When the aqueous fluoride concentration exceeded 100 ppm, the total amount of fluoride adsorbed onto hydroxyapatite decreased after being washed off with water three times. It is postulated that when the aqueous fluoride concentration exceeds 100 ppm, a multilayer structure of calcium ...

This study introduces the application of calcium-derivatized hydroxyapatite for purification of Fab. Fab binds to native hydroxyapatite but fails to bind to the calcium derivatized form. IgG, Fc, and most other protein contaminants bind to the calcium form. This supports Fab purification by a simple flow-through method that achieves greater than 95% purity from papain digests and mammalian cell...