Abstract Background While conventional frozen sections and other ex vivo microscopy always come along with artefacts, in confocal laser endomicroscopy (CLE) offers less invasive, manipulated imaging of living tissues. Understanding the distribution staining agent sodium-fluorescein (NaFl) plays a key role establishing CLE as new opportunity for real-time transmitted, imaging. By comparing NaFl ...

Fluorescein is one of most used fluorescent labels for characterizing biological systems, such as proteins, and is used in fluorescence microscopy. However, if fluorescein is to be used for quantitative measurements involving proteins then one must account for the fact that the fluorescence of fluorescein-labeled protein can be affected by the presence of intrinsic amino acids residues, such as...

BACKGROUND Sentinel lymph node biopsy (SLNB) is well accepted to be a standard procedure in breast cancer surgery with clinically negative lymph nodes. Isosulfan blue is the first dye approved by the USA Food and Drug Administration for the localization of the lymphatic system. Few alternative tracers have been investigated. In this study, we aimed to compare the differences between 10 % fluore...

This paper discusses our experience with oral fluorescein in general and specifically its use on a group of Ehlers-Danlos patients. I will discuss our methods and results.

A slit-lamp fluorophotometric method is presented that permits calculation of a blood-retinal barrier permeability to fluorescein (P) and a diffusion coefficient for fluorescein in the vitreous body (D). The calculations are performed by relating the time course of the free--not protein bound--fluorescein concentration in the bloodstream with the fluorescein concentration profile in the vitreou...

The quantum yield of singlet oxygen production was investigated in dodecylammonium propionate reversed micellar solutions containing 1,3-diphenylisobenzofuran and fluorescein as photosensitizer. The quantum yield was highly enhanced by the binding of fluorescein to reversed micelles, and increased with decreasing the solubilized water content in reversed micelles. Results obtained was discussed...

Gold nanoparticles (GNPs) protected by poly(2-ethyl-2-oxazoline) (POZ) of different molecular weights (Mw = 5, 50, 200 and 500 kDa) were synthesised characterised dynamic light scattering, nanoparticle tracking analysis, zeta potential measurement transmission electron microscopy. It was established that the use POZ with 50 kDa resulted in formation GNPs low polydispersity while greater led to ...