The chemical nature of the non-tryptophan (non-Trp) fluorescence of porcine and human eye lens proteins was identified by Mass Spectrometry (MS) and Fluorescence Steady-State and Lifetime spectroscopy as post-translational modifications (PTM) of Trp and Arg amino acid residues. Fluorescence intensity profiles measured along the optical axis of human eye lenses with age-related nuclear cataract ...

BACKGROUND AND OBJECTIVE The hamster cheek pouch carcinogenesis model, using chronic treatments of dimethylbenz[alpha]anthracene (DMBA) was used as a model system to investigate changes in epithelial tissue autofluorescence throughout the dysplasia-carcinoma sequence. STUDY DESIGN/MATERIALS AND METHODS Fluorescence emission spectra were measured weekly from 42 DMBA-treated animals and 20 cont...

The effects of aspirin, vitamin B(12) and warfarin as potential blockers of the ruthenium binding sites in HSA were investigated through UV/visible, circular dichroism (CD), fluorescence spectroscopy and the inductively coupled plasma-atomic emission spectroscopy ICP(AES). The studies on the interactions of several biologically relevant molecules with HSA have shown that drugs like aspirin or w...

Excitation–emission matrix (EEM) fluorescence spectroscopy was employed to study the chemical nature of organic matter readily released into solution from sediment particles during episodes of resuspension. Surface sediment samples collected from five locations within the Hudson River Estuary and the Inner New York Bight were resuspended in their respective bottom waters for periods ranging fro...

The phototropins are blue-light receptors that base their light-dependent action on the reversible formation of a covalent bond between a flavin mononucleotide (FMN) cofactor and a conserved cysteine in light, oxygen or voltage (LOV) domains. The primary reactions of the Avena sativa phototropin 1 LOV2 domain were investigated by means of time-resolved and low-temperature fluorescence spectrosc...

This review covers the field of excited electronic-state chemical reactions in small clusters. The clusters emphasized are those comprised of an organic chromophore that is electronically excited to initiate the reaction and of various coreactant molecules ranging from water and ammonia to ethers, amines, aromatics, alkanes, alkenes, and diatomics. The reactions discussed include vibrational re...

CdSe/CdS colloidal nanocrystals are members of a novel class of light-emitting nanoparticles with remarkable optical properties such as suppressed fluorescence blinking and enhanced emission from multiexciton states. These properties have been linked to the suppression of non-radiative Auger recombination. In this work we employ ultrafast spectroscopy techniques to identify optical signatures o...

We report on a fiber laser-based stimulated emission-depletion microscope providing down to ∼20 nm resolution in raw data images as well as 15-19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. Th...

As continuation of previous studies, the ability of T-284 and SH-516 cyanine dyes (Figure 1) to selectively detect fibrillar proteins of various amino acids composition (insulin, lysozyme, αsynuclien) was evaluated using fluorescence spectroscopy methods (Table). Both dyes were shown to be applicable for detection of various fibrillar protein species (Figure 2). T-284 demonstrated particular se...

This paper introduces recent optical measurements of radicals in atmospheric pressure non-thermal plasma. Laser-induced fluorescence (LIF), laser absorption, and optical emission spectroscopy (OES) techniques have been applied for density and temperature measurements of OH radicals, NO molecules, ozone, atomic oxygen, atomic nitrogen, and N2( A3Σ u ) metastables in pulsed corona and pulsed diel...