نتایج جستجو برای: fluorometric assay
تعداد نتایج: 223370 فیلتر نتایج به سال:
Photoactivated hypericin has a potent cytotoxic effect over a wide range of cells. However, very recently hypericin has been shown to have antitumoral and antimetastatic effects in the dark. The aim of this study was to test whether hypericin in the dark affects angiogenesis. Different in vitro assays were used to study the potential effects of this compound on key steps of angiogenesis, namely...
We have performed a one-step all-in-one dual-label dry reagent time-resolved fluorometric assay measuring free and total prostate-specific antigen (PSA). All assay-specific components were predried in an ordinary microtitration well, including the Eu and Tb chelate-labeled monoclonal antibodies for the recognition of free and total PSA, respectively. Only the sample and assay buffer needed to b...
Background and Objective: Oxidative stress plays an important role in the pathogensis of various diseases, including lung cancer, chronic obstructive pulmonary disease, and atherosclerosis. Total antioxidant capacity plays a significant role in the body’s antioxidant defense, so its assessment is a matter of the utmost importance. Currently, assessment of this capacity is performed in var...
Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Amplex Red. HDLox correlated with previously validated cell-based (r = 0.47, p<0.001) and cell-free...
Gene diagnosis shows tremendous promise for medical, pharmaceutical, and other applications. The most reliable method for detection of single-nucleotide polymorphisms (SNPs) is direct sequencing. However, this approach suffers from high cost and low throughput. Instead of the direct sequencing, various assays have been developed. Especially, combination of the allele-specific hybridization and ...
A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The rate of formation of 3-chloro-7-hydroxy-4-methylcoumarin was recorded as an increase of fluorescence (lambdaA = 380 nm, lambdaF = 480 nm) with...
PURPOSE Mucopolysaccharidosis type II (MPS II or Hunter syndrome) is a rare lysosomal storage disorder caused by iduronate-2-sulfatase (IDS) deficiency. MPS II causes a wide phenotypic spectrum of symptoms ranging from mild to severe. IDS activity, which is measured in leukocyte pellets or fibroblasts, was reported to be related to clinical phenotype by Sukegawa-Hayasaka et al. Measurement of r...
Several different types of assay procedure have been proposed for the quantitative determination of thiamine. In a discussion (1) of the difficulties encountered in some of the methods, it has been pointed out that calorimetric (2, 3) and fluorometric (4) methods often suffer from a lack of sufficient sensitivity or specificity. The procedure described in the present communication, while not so...
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