Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

death is one of the main issues in samih ghasem works; because he paid special attention to it in his works. the present paper studies his ideologies about death as well as his motivations on expressing this issue. his aesthetic view strengthens resistance spirit in his hometown fellows and causes changing in their view point on death. this article has been done in descriptive – analytic method...

Methodical approaches to the study of lyrics in literature lessons are disclosed article, particular, its role formation literary competence students general secondary education institutions. It is noted that most mobile all genres and have greatest potential shaping emotional sphere great educational opportunities. Various methodical algorithms for lyric poetry analyzed, fact divided into civi...

narrative style is the main feature of mehdi akhavan sales (1928-1990), one of the famous contemporary persian poets. but what gives a special prominence to his narrative poems and makes it distinct from the works of other contemporary poets is his idiosyncratic use of narrative techniques and methods. he employed in his works the element of narration in a distinctive manner. although, the narr...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...