For extracting free amino acids from human leukocytes, we find that disruption of the cells by ultrasonication is more reliable than freezing and thawing. The amount of free amino acids extracted by the latter method depends on the temperature and duration of thawing. We extracted free amino acids from leukocytes of healthy men by ultrasonication and compared their concentrations in lymphocytes...

Administration of 9a-fluoroprednisolone causes an early (3 hr) decrease of basic amino acid incorporation into CS-P1798 lymphosarcoma protein. Decreased utilization of neutral amino acids is observed after 6 hr. These effects are accompanied by expansion of the intratumoral free amino acid pool in CS-but not in CR-P1798. Thus, decreased incorporation of radioactive amino acids in CS-P1798 appea...

Administration of 9a-fluoroprednisolone causes an early (3 hr) decrease of basic amino acid incorporation into CS-P1798 lymphosarcoma protein. Decreased utilization of neutral amino acids is observed after 6 hr. These effects are accompanied by expansion of the intratumoral free amino acid pool in CS-but not in CR-P1798. Thus, decreased incorporation of radioactive amino acids in CS-P1798 appea...

A method for quantitative analysis of free amino acids in rat brain was established. Rat midbrain was homogenized in HC104 solution and the supernatant was applied to a high-performance liquid chromatograph attached to a fluorescence detector. Amino acids were separated in an ion exchange column prepared for amino acid analysis and were reacted with o-phthalaldehyde. Fluorescence intensity was ...

Isoelectric solubilization /precipitation (ISP) process was used to isolate protein from muscles of Prussian carp, Carassius gibelio (Bloch, 1782). Fish protein and lipid were recovered from whole gutted Prussian carp using acidic and basic isoelectric solubilization/precipitation followed by assaying amino acid and fatty acid profile. Essential amino acids content in acidic and basic pH treatm...

Protein hydrolysis to peptides and free amino acids and the apparent absorption of amino acids (AAaa) were evaluated in different segments of carp intestine. The AAaa analysed using Cr2O3 as a marker indicated that 73.2% of the amino acids were absorbed in the first 20% of the intestinal tract and 5.3 and 21.5, respectively, in the following segments (20% of gut length). Except for methionine a...