objective (s): the scarcity of articular cartilage defect to repair due to absence of blood vessels and tissue engineering is one of the promising approaches for cartilage regeneration. the objective of this study was to prepare an extracellular matrix derived decellularized bovine articular cartilage scaffold and investigate its interactions with seeded rat bone marrow mesenchymal stem cells (...

Microbial activity is known to continue during the winter months in cold alpine and Arctic soils often resulting in high microbial biomass. Complex soil nutrient dynamics characterize the transition when soil temperatures approach and exceed 0 1C in spring. At the time of this transition in alphine soils microbial biomass declines dramatically together with soil pools of available nutrients. Th...

Under the complex working conditions in cold areas, order to achieve health monitoring of engineering structures, carbon fiber and iron tailings sand were added ordinary cement-based materials prepare piezoelectric composites, deterioration their pressure-sensitive properties mechanical under action sulfate-freeze-thaw cycle was studied. Six groups specimens a set benchmark prepared according d...

The use of superabsorbent polymers (SAPs) to produce internally cured concrete has been shown to be effective in reducing the potential for restrained shrinkage cracking of high performance concrete mixtures. However, not much is known regarding the freeze–thaw durability of concrete mixtures that incorporate SAPs for internal curing (IC). When SAP particles desorb (or partially desorb) the “wa...

This paper summarizes a series of studies on the interface of fiber reinforced polymer laminates externally bonded to concrete substrates, carried out in the Laboratory of Engineering for Maintenance Systems, Hokkaido University. Appropriate test methods and bond models for shear bond, tensile bond and cleavage bond (bond under dowel force) are shown. Finally, experimental results on shear bond...

Treponema pallidum DNA from even small numbers of organisms was detectable in cerebrospinal fluid (CSF) stored at room temperature or at 4 degrees C for several hours and in CSF subjected to three freeze-thaw cycles. These results suggest that negative PCR results for T. pallidum from patients diagnosed with T. pallidum invasion of the central nervous system are probably not due to the loss of ...

The mechanical deterioration of soft rocks under freeze–thaw cycles is caused by the accumulation mesoscopic damage. However, current model for does not adequately consider multiscale correlations, which makes strength calculation results differ greatly from test and cannot fully reveal damage mechanism cycling conditions. In this paper, bond pore ice expansion laws are considered soft-rock uni...

An enzyme immunoassay producing either a chromogenic or fluorogenic end product was developed and evaluated for detecting La Crosse viral antigen within mosquito pools. The enzyme immunoassay was found to be sensitive, detecting one infected mosquito within a pool of 100 mosquitoes, and specific, distinguishing between closely related California group viruses. Assays were completed within 5 h a...

Soil structure plays a central role in many soil processes that are environmentally relevant. Intermittent freezing of the over winter is an important abiotic disturbance temperate climates and its effects on depend soil’s preexistent structural strength cohesion. Management choices such as tillage plant cover after harvest strongly influence structure, therefore response to freeze-thaw. We exa...

A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro ...