Single-cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at the single cell level and has not been reported for equine sperm. The ability to detect nuclear damage at the single cell level could aid in the advancement of protocols for optimal semen preservation. The goals of these experiments were to adapt this assay for use with equine sperm and to utilize the as...

A fertility trial was conducted comparing two methods of defining the optimum time to inseminate frozen boar semen. One hundred sixty-four gilts were inseminated, one-half at a fixed insemination time of 32 to 34 h after calculated onset of estrus and the remainder were inseminated on the basis of changes in the vaginal mucosal electrical resistance. Inseminations were made when the instrument ...

Indonesia has two National Artificial Insemination Centers (AIC) and more than 15 Regional (RAIC) spread across several provinces. Bulls in the AIC must have a high libido produce good quality semen. This study examines correlation between with semen testosterone concentration to determine potential frozen production from Bali bulls South Sulawesi RAIC. Ten bull were used this study. Semen coll...

As an attempt to find an inexpensive and simple laboratory method for artificial insemination (AI) bull semen quality assessment, the osmotic resistance of spermatozoa was measured using the hypoosmotic swelling (HOS) test, developed by Jeyendran et al. (1984) (labelled HOS-1), and its modifications (HOS-2, HOS-3), with decreased osmotic pressure aimed at challenging sperm survival ability. The...

The current study investigated the relationship between the level of expression and tyrosine phosphorylation of the sperm protein 32 (sp32) and the activation of the boar proacrosin/acrosin system. The acrosomal membrane proteins of boar sperm for use in different treatment experiments (i.e., fresh sperm, freezing and thawing, capacitation, and acrosome reaction) were separated, stained by Coom...

Our objective was to identify predictors of improved postthaw semen quality in men with testicular cancer banking sperm for fertility preservation. We reviewed 173 individual semen samples provided by 67 men with testicular germ cell tumor (TGCT) who cryopreserved sperm before gonadotoxic treatment between 1994 and 2010 at our tertiary university medical center. Our main outcomes measures were ...

Different semen assays were employed to determine the post -thaw fitness of buffalo and Sahiwal bull spermatozoa. Semen (two ejaculates) was collected from buffalo and Sahiwal bulls (six each) once a week for three consecutive weeks. Semen was diluted in lactose-fructose-egg yolk glycerol extender, equilibrated at 4 ac for 4 hr and frozen in liquid nitrogen vapors. A highly significant differen...

The present study investigated the possibility of replacing salamon with modified shotor diluent (MSD) and egg yolk (EY) with low density lipoprotein (LDL) for chilled storage of ram semen. Good quality semen (>80% progressive forward motility (PFM) of sperm) from 3 fertile rams was collected using an artificial vagina and pooled for each experiment. Low density lipoprotein was extracted from f...

Artificial insemination is a well-established and widely used method for genetic improvement in cattle breeding industry. Recently, researchers have shown an increased interest in the cryoprotective effects of minerals and antioxidants on semen. Previous studies on calcium (Ca) and magnesium (Mg), two main macro-minerals, have mainly investigated their roles in mammalian spermatogenesis and fer...

In sexual assault cases, particularly those involving internal child sex trafficking (ICST), victims often hide their semen-stained clothing. This can result in a lag time of several months before the items are laundered and subsequently seized during a criminal investigation. Although it has been demonstrated previously that DNA can be recovered from clothing washed immediately after semen dep...