alpha-L-Arabinofuranosidase (alpha-L-arafase) was purified from fruit of Japanese pear (Pyrus pyrifolia). The enzyme solubilized from the cell wall by NaCl and Triton X-100 had the homogeneity of a single 62-kD polypeptide on SDS-PAGE after purification through the steps of hydroxyapatite, anion-exchange chromatography, and size-exclusion chromatography. A related cDNA clone was isolated (PpARF...

Expansins are proteins that induce extension in isolated plant cell walls in vitro and have been proposed to disrupt noncovalent interactions between hemicellulose and cellulose microfibrils. Because the plant primary cell wall acts as a constraint to cell enlargement, this process may be integral to plant cell expansion, and studies of expansins have focused on their role in growth. We report ...

The quality of fruit targeted for human consumption is affected greatly by the methods used in processing. The objective of this study was to assess a method of treating cantaloupe melons to reduce the wound responses incurred by cutting while maintaining fruit quality. After being treated with an alternating magnetic field (AMF) at the strength of 2mT for 0, 5, 10, 15, 20, or 25 min, Cucumis m...

The effects of suppressing deoxyhypusine synthase (DHS) have been examined in tomato (Solanum lycopersicum cv UCT5). DHS mediates the first of two sequential enzymatic reactions that activate eukaryotic translation initiation factor-5A (eIF-5A) by converting a conserved Lys to the unusual amino acid, deoxyhypusine. DHS protein levels were suppressed in transgenic plants by expressing the 3'-unt...

Fruit softening is an important part of the ripening process, and involves changes to cell turgor and primary cell wall structure. Most of the polysaccharide components of the cell wall are subjected to some degree of controlled degradation, resulting in a loosening and swelling of the wall structure, a weakening of cell wall strength, and reduced intercellular adhesion. Early ripening changes ...

Pectins are essential components of primary plant cell walls and middle lamellae, and are related to the consistency of the fruit and its textural changes during ripening. In fact, strawberries become soft as the middle lamellae of cortical parenchyma cells are extensively degraded during ripening, leading to the observed short post-harvest shelf life. Using a custom-made oligonucleotide-based ...