Histopathology archives represent a vast source of infectious disease specimens that can be used to elucidate important disease processes. In this report, we describe a method to detect Candida albicans gene expression from infected, formalin-fixed, paraffin-embedded mouse tissue. By use of glass beads to break open the fungal cells and proteinase K treatment, RNA was extracted routinely from t...

we present a case of allergic fungal sinusitis (afb) in a 20-year old man with few months' history of bilateral nasal obstruction and discharge with unilateral proptosis that underwent maxillary antrostomy due to the mass in paranasal sinuses. histological examination of tissue showed branching fungal hyphae interspersed with allergic mucin without fungal invasion to soft tissue. the patient re...

fusarium head blight (fhb) caused by fusarium graminearum is responsible for billions of dollars in agriculture losses. the goal of the present study was evaluation the expression of acidic chitinase, one of pr proteins, in wheat defense response against different fhb induced treatments in 'falat' as a highly susceptible and 'sumai3' as a tolerant cultivar. these treatments contained fungi extr...

Among the endemic deep mycoses in Latin America, paracoccidioidomycosis (PCM), caused by thermodimorphic fungi of the Paracoccidioides genus, is a major cause of morbidity. Disease development and its manifestations are associated with both host and fungal factors. Concerning the latter, several recent studies have employed the methodology of gene modulation in P. brasiliensis using antisense R...

Within the last two decades, the incidence of invasive fungal infections has been significantly increased. They are characterized by high mortality rates and are often caused by Candida albicans and Aspergillus fumigatus. The increasing number of infections underlines the necessity for additional anti-fungal therapies, which require extended knowledge of gene regulations during fungal infection...

Fungal species Lasiodiplodia theobromae of family Botryosphaeriaceae is a pathogen causing significant loss production and quality mango crop in Vietnam worldwide. In this study, fungal strain designed as XB1 was isolated from stem-end rot fruits, identified tested its susceptibility to silver copper nanoparticles. Strain identification based on biological characteristics sequence analysis 5.8S...

Black stain is a postharvest disease that occurs on the skin of Niitaka pears (Pyrus pyrifolia Nakai) during cold storage, causing significant economic losses to pear production. We investigated antifungal effects peracetic acid (PAA), which strong oxidizing agent and an effective disinfectant, fungi cause black stain, we confirmed its preventive effect after application pears. Fungal isolates ...

A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the P. ostreatus ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp ...

The reexamination of the fungal genus Botryosphaeria on 12 plant species 10 families was carried out based molecular phylogenetic analyses using regions translation elongation factor 1-α, β-tubulin, DNA-directed RNA polymerase II subunit, and internal transcribed spacer region rDNA morphological characteristics. Japanese isolates were divided into five clades include dothidea, B. qingyuanensis,...