During cytokinesis, a specialized set of proteins is recruited to the equatorial region between spindle poles by microtubules and actin filaments, enabling furrow assembly and ingression before cell division. We investigate the mechanisms underlying regional specialization of the cytoskeleton in HeLa cells undergoing drug-synchronized monopolar cytokinesis. After forced mitotic exit, the cytosk...

During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation o...

Cleavage furrow formation in animal cells results from a local increase in cortical contractility. During anaphase, the spindle contains, in addition to astral arrays of microtubules, a set of bundled microtubules known as the central spindle. Each of these populations of microtubules, the astral arrays and the central spindle bundles, is sufficient to direct cleavage furrow formation, yet in w...

There is currently a limited understanding of the complex response fungal microbiota diversity to organic fertigation. In this work, 2-year field trial with tomato crops in soil previously amended fresh sheep manure was conducted. Two hypotheses were compared: (i) fertigation liquid fertilizers versus (ii) irrigation water. At end both years, soils analyzed for physical–chemical parameters and ...

Experiments on model systems have revealed that cytokinesis in cells with contractile rings (amoebas, fungi, and animals) depends on shared molecular mechanisms in spite of some differences that emerged during a billion years of divergent evolution. Understanding these fundamental mechanisms depends on identifying the participating proteins and characterizing the mechanisms that position the fu...

Many of the same genes needed for proper eye and limb development in vertebrates, such as hairy, hedgehog, patched and cyclic AMP-dependent protein kinase A, are responsible for patterning Drosophila imaginal discs, the tissues that will give rise to the adult cuticle structures. This is well demonstrated in the control of morphogenetic furrow movement and differentiation in the eye imaginal di...

ABSTRACT: Fertigation is widely used in seedling production for different crops, including passion fruit. There no consensus on the ideal nutrient solution, and as such, nurseries have fertigation empirically. This study assessed electrical conductivity of solution containing macro micronutrients fruit compared it with commercially fertigation. A completely randomized design was used, five trea...

Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other ...

in order to improve the efficiency of irrigation systems and investigate their impact on the pest population levels, furrow and sprinkler irrigation systems were compared in sugar beet fields. in this study, three different fields were chosen and irrigated by one of the furrow, classic and center pivot irrigation systems.  in each field, 20 plots were randomly selected of which 10 plots were ma...