نتایج جستجو برای: gag gene

تعداد نتایج: 1146521  

Journal: :The Journal of Experimental Medicine 1989
J P Klarnet D E Kern K Okuno C Holt F Lilly P D Greenberg

Immunization of C57BL/6 (B6) mice with FBL, a Friend murine leukemia virus (F-MuLV), induces both tumor-specific cytolytic CD8+ (CTL) and lymphokine-producing CD4+ Th that are effective in adoptive therapy of B6 mice bearing disseminated FBL leukemia. The current study evaluated the F-MuLV antigenic determinants expressed on FBL that are recognized by FBL-reactive CD8+ and CD4+ T cells. To iden...

Journal: :Genes & genetic systems 1999
H Ohtsubo N Kumekawa E Ohtsubo

The 441-bp DNA segment in a PCR-amplified fragment from Oryza sativa cv. IR36 was found to have a sequence with features characteristic of LTRs of retroelements, which was named RIRE2 (Rice retroelement #2) and further analyzed. Cloning and sequencing analyses of the DNA segments connected to LTR-like sequence showed that RIRE2 has a long internal region almost 10 kb long that is flanked by LTR...

Journal: :Journal of virology 2003
Danilo R Casimiro Ling Chen Tong-Ming Fu Robert K Evans Michael J Caulfield Mary-Ellen Davies Aimin Tang Minchun Chen Lingyi Huang Virginia Harris Daniel C Freed Keith A Wilson Sheri Dubey De-Min Zhu Denise Nawrocki Henryk Mach Robert Troutman Lynne Isopi Donna Williams William Hurni Zheng Xu Jeffrey G Smith Su Wang Xu Liu Liming Guan Romnie Long Wendy Trigona Gwendolyn J Heidecker Helen C Perry Natasha Persaud Timothy J Toner Qin Su Xiaoping Liang Rima Youil Michael Chastain Andrew J Bett David B Volkin Emilio A Emini John W Shiver

Cellular immune responses, particularly those associated with CD3(+) CD8(+) cytotoxic T lymphocytes (CTL), play a primary role in controlling viral infection, including persistent infection with human immunodeficiency virus type 1 (HIV-1). Accordingly, recent HIV-1 vaccine research efforts have focused on establishing the optimal means of eliciting such antiviral CTL immune responses. We evalua...

2016
Christina D. Eckstrand Chadwick Hillman Brian G. Murphy

Feline immunodeficiency virus (FIV) infection results in viral persistence, a prolonged asymptomatic phase, and progressive immunopathology. During the asymptomatic phase, a cohort of experimentally FIV-infected cats exhibits features of viral latency in blood suggestive of inactive viral replication. We sought to investigate viral replication activity and genomic stability of the FIV proviral ...

Journal: :Viruses 2016
Melanie Friedrich Christian Setz Friedrich Hahn Alina Matthaei Kirsten Fraedrich Pia Rauch Petra Henklein Maximilian Traxdorf Torgils Fossen Ulrich Schubert

The HIV-1 Gag p6 protein regulates the final abscission step of nascent virions from the cell membrane by the action of its two late (L-) domains, which recruit Tsg101 and ALIX, components of the ESCRT system. Even though p6 consists of only 52 amino acids, it is encoded by one of the most polymorphic regions of the HIV-1 gag gene and undergoes various posttranslational modifications including ...

Journal: :The Journal of Experimental Medicine 2008
Paul A. Goepfert Wendy Lumm Paul Farmer Philippa Matthews Andrew Prendergast Jonathan M. Carlson Cynthia A. Derdeyn Jianming Tang Richard A. Kaslow Anju Bansal Karina Yusim David Heckerman Joseph Mulenga Susan Allen Philip J.R. Goulder Eric Hunter

In a study of 114 epidemiologically linked Zambian transmission pairs, we evaluated the impact of human leukocyte antigen class I (HLA-I)-associated amino acid polymorphisms, presumed to reflect cytotoxic T lymphocyte (CTL) escape in Gag and Nef of the virus transmitted from the chronically infected donor, on the plasma viral load (VL) in matched recipients 6 mo after infection. CTL escape muta...

Journal: :The Journal of clinical investigation 2007
Shih-Wen Lin Scott E Hensley Nia Tatsis Marcio O Lasaro Hildegund C J Ertl

Recombinant adeno-associated virus (rAAV) vectors were used in human trials as carriers of vaccines for HIV-1 after encouraging preclinical results. However, the clinical trials yielded disappointing results. Here we demonstrated that in mice, rAAV vectors expressing the gene encoding HIV-1 gag stimulated gag-specific CD8(+) T cells, but these T cells failed to expand after a booster immunizati...

Journal: :Journal of virology 1991
R A Littaua M B Oldstone A Takeda C Debouck J T Wong C U Tuazon B Moss F Kievits F A Ennis

A unique epitope on the gag protein of human immunodeficiency virus type 1 (HIV-1), located at amino acid 145 to 150, has been mapped by using a CD8+ cytotoxic T-lymphocyte (CTL) clone. This epitope is highly conserved among 18 HIV-1 strains. The HIV-1 gag-specific human leukocyte antigen (HLA) class I-restricted CD8+ CTL clone was generated from fresh peripheral blood mononuclear cells of an H...

Journal: :Journal of virology 2009
Andreas Leiherer Christine Ludwig Ralf Wagner

Apart from its regulatory role in protease (PR) activation, little is known about the function of the human immunodeficiency virus type 1 transframe protein p6* in the virus life cycle. p6* is located between the nucleocapsid and PR domains in the Gag-Pol polyprotein precursor and is cleaved by PR during viral maturation. We have recently reported that the central region of p6* can be extensive...

2013
FangZheng Li LianBing Li Ying Zhong QingDong Xie JiHua Huang XiangJin Kang Dian Wang Lan Xu TianHua Huang

OBJECTIVE Studying the methylation status of long terminal repeats (LTR) and its relationship to gag expression of HIV-1 in order to explore regulation mechanism of HIV-1 gene expression in vertical transmission from sperm to embryo. METHODS/PRINCIPAL FINDINGS Sperm samples were collected from a healthy donor and seven patients with HIV/AIDS. Zona-free hamster ova were fertilized by donor's s...

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