The improvement in hyphenated analytical techniques has significantly widened their applications to the analysis of biomaterials. In this article, we discuss recent advances in applications of hyphenated chromatographic techniques including capillary electrophoresis to the analyses of biological samples. As tools of separation, gas chromatography, high-performance liquid chromatography and capi...

Glycation of immunoglobulin G (IgG) can result from incubation with a reducing sugar in vitro or during circulation in vivo. Upon injection of a recombinantly produced human therapeutic IgG into humans, changes in the glycation levels could be observed as a function of circulation time. Mass changes on the individual IgG polypeptide chains as the results of glycation were determined using rever...

A gas chromatographic method of pregnanediol determination has been carried out on 24-hr. urine specimens collected during various phases of the menstrual cycle and during pregnancy. Pregnanediol values were correlated with endometrial histology and cycle day in nonpregnant subjects, and with duration of gestation during pregnancy. Determinations were performed on free pregnanediol and on the a...

N,N'-Methylenebisacrylamide (MBAA), which is an important raw material in a widely industrial area, was synthesized using convenient catalysts. The synthesized monomer was fully characterized by the elemental analysis, fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), 1H-NMR, 13C-NMR, GC/MS, and high performance liquid chromatography (HPLC) analysis. Six differe...

Protected deoxyribonucleotides were identified by field desorption mass spectrometry in eluate fractions from high performance liquid chromatography. The production of abundant cationized molecules and the formation of structural significant fragments by thermally/field induced processes allowed a direct and unambiguous identification of the synthesized products. In addition, indications can be...

E. C. HORNING, Baylor University College of Medicine, Houston, Teras (Chairman) E. H. AHRENS, JR., The Rockefeller Institute, New York, New York S. R. LIPSKY, Yale University School of Medicine, New Haven, Connecticut F. H. MATTSON, Procter and Gamble Co., Cincinnati, Ohio J. F. MEAD, School of Medicine, University of California at Los Angeles, Los Angeles, California D. A. TURNER, Sinai Hospit...

In the present study we used chiral high performance liquid chromatography and gas chromatography to determine the stereoisomers of racemic alpha-tocopherol. The Chiralcel OD-H and Silar-100 AT columns were used. Statistical evaluation of the results established the precision of these methods.

Radix Salviae miltiorrhizae et Rhizoma, known as Danshen in China, is one of the most popular traditional Chinese medicines. Recently, there has been increasing scientific attention on Danshen for its remarkable bioactivities, such as promoting blood circulation, removing blood stasis, and clearing away heat. This review summarized the advances in chemical analysis of Danshen and its preparatio...

Abbreviations and trivial names: chenodeoxycholate, 3a,7adihydroxy-5p-cholanate; TLC, thin-layer chromatography; PLC, preparative-layer chromatography; HPLC, high-pressure liquid chromatography; PMR, proton magnetic resonance spectroscopy; GLC, gas-liquid chromatography; MS, mass spectrometry. Address correspondence to William H. Elliott, E. A. Doisy Department of Biochemistry, St. Louis Univer...