Proteose peptone (PP) is a heat-stable and acid-soluble protein in milk whey. We reveal in this study the IgE production-suppressing activity of the PP fraction in bovine milk. The PP fraction suppressed IgE production by human myeloma cell line U266 cells by depressing the IgE mRNA expression. The suppressive activity of the PP fraction was facilitated by trypsin digestion. An oral administrat...

In the course of studies on the acid-soluble phosphorus compounds of liver and kidney, it became evident that the fraction comprising the nucleotides represented a complex mixture (1). In an attempt to isolate the individual nucleotides, methods designed to minimize the breakdown of labile phosphorus compounds were employed. The isolation of adenylic acid and of a material representing a mixtur...

Chromatin from trout testis at an early stage of development was digested with DNase II (deoxyribonucleate 3'-oligonucleotidohydrolase; EC 3.1.4.6), and the solubilized products were fractionated into Mg2+-soluble and -insoluble components. An examination of the histones from these fractions by one- and two-dimensional polyacrylamide gels showed that the highly acetylated species of histone H4 ...

Insulin has been shown to be composed oftwo types of polypeptide chains held together by -S-bridges. These chains may be separated after breaking the -S-Sbridges by oxidation to -SOH groups (Sanger, 1949 a). The more acidic glycyl chain is found in the fraction (A) which is soluble at pH 6-5 and the more basic phenylalanyl chain in the insoluble fraction (B). Bymaking use ofthe methods of Consd...

Diets containing freeze-dried black raspberries (BRB) suppress the development of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Using bioassay-directed fractionation, the anthocyanins in BRB were found to be the most active constituents for down-regulation of carcinogen-induced nuclear factor-kappaB and activator protein-1 expression in mouse epidermal cells in vitro. T...

In methanogenic microbial electrolysis cells (MMCs), CO2 is reduced to methane using a methanogenic biofilm on the cathode by either direct electron transfer or evolved hydrogen. To optimize methane generation, we examined several cathode materials: plain graphite blocks, graphite blocks coated with carbon black or carbon black containing metals (platinum, stainless steel or nickel) or insolubl...

Alkaline hydrolysis of the ether-insoluble resin glycoside (convolvulin) fraction of the seeds of Quamoclit pennata BOJER (Convolvulaceae) provided five new glycosidic acids, quamoclinic acids B, C, D, E, and F, along with six organic acids, isobutyric, 2S-methylbutyric, tiglic, 2R,3R-nilic, 7S-hydroxydecanoic, and 7S-hydroxydodecanoic acids. These new compounds were characterized on the basis ...

objective eudragits are widely used polymers in the production of oral sustained release dosage forms. the application of these polymers in the production of inert insoluble matrices has been investigated. however the effect of particle size, compaction force and presence of aerosil 200 as a glidant on the properties of eudragit rs and rl matrices prepared by direct compression of their physica...

the aim of the current study was to evaluate the replacement effect of soybean meal (sbm) at different levels (0 as d1, 33 as d2, 67 as d3 and 100% as d4 based on crude protein (cp) content) by guar meal (gm) on ruminal gas production kinetics, ruminal digestibility and fermentation characteristics of a fattening diet for lambs. three experiments were conducted. the first experiment of 144-h in...

The effects of whole wheat bran and its components on the absorption of nonheme dietary iron were measured using a double isotope technique in human volunteers. When 12 g bran was added to a light meal, absorption decreased by 51 to 74%; this inhibitory effect of bran was shown for meals of both high and low iron availability. Inhibition was not explained by monoferric phytate, the major form o...