The first paper of this series (1) described an electrophoretic demonstration of a complex between pepsin and bovine serum albumin during the course of the enzymatic hydrolysis of the latter. Several independent lines of evidence were presented that this complex is the Michaelis-Menten complex capable of being activated to give rise to reaction products. This communication reports the results o...

A study of the kinetics of the transformation of swine pepsinogen into pepsin under a variety of conditions has been made. The results show that the transformation as a whole is essentially autocatalytic in nature under all conditions. Evidence is presented to show the existence of a compound intermediate between pepsinogen and pepsin. This compound was found to be a reversibly dissociable comp...

Kinoshita, Mine, Eisuke Kume, Shigeki Igarashi, Nobuko Saito, and Hiroshi Narita. Role of salivary mucin in the protection of rat esophageal mucosa from acid and pepsin-induced injury. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G796–G800, 1999.—The mucosal defensive mechanisms of the esophagus against acid and pepsin remain to be elucidated. In the present study, we investigated the...

Acid gastro-oesophageal reflux (GOR) and gastric aspiration have been labelled as risk factors for chronic rejection bronchiolitis obliterans syndrome (BOS) after lung transplantation (LTx). The present study aimed to further characterise GOR (both acid and nonacid) and the degree of gastric aspiration in LTx recipients both with and without BOS. Impedance-pH recordings were used for GOR detect...

1. A method is described for the preparation of pepsinogen from swine gastric mucosae which consists of extraction and fractional precipitation with ammonium sulfate solutions followed by two precipitations with a copper hydroxide reagent under particular conditions. Crystallization as very thin needles takes place at 10 degrees C., pH 5.0 and from 0.4 saturated ammonium sulfate solution contai...

The objective of this study was to investigate the yield and characteristics collagen protein extracted from muscle four different species mantis shrimp: Miyakella nepa, Harpiosquilla harpax, Erugosquilla woodmasoni, Odontodactylus cultrifer. Mantis shrimp by using a pepsin-solubilization technique, with 0.5 M acetic acid 5% pepsin enzyme. highest M. nepa (0.478 ± 0.06%), which significantly gr...

Isolation of mono-iodotyrosine from slightly iodinated pepsin has been repeated and the properties of the product compared with those of synthetic dl-3-iodotyrosine. Ultraviolet absorption spectra, pK values of the phenol group, solubility measurements, and partition coefficients were so nearly identical for the two materials that there is now no reason to doubt that the product from pepsin is ...