A sensitive technique for plasma insulin assay based on displacement of labeled insulin from its antibody was reported by y ALLOW and BERSON in 1960 (9). Subsequently, similar methods based on competitive protein binding have been deve1oped. In 1963 MURPHY and co-workers (6) described a technique involving a dialysis using 14C-cortisol. The time required for this procedure was shortened from tw...

Glucokinase is a monomeric protein under native and denaturating conditions yet presents a sigmoidal saturation function for glucose. These peculiarities suggested the possibility that polymerization occurs under assay conditions. Thus the apparent molecular weight of glucokinase was determined by gel filtration at 4°C and at 30°C in the presence of substrates and products, singly and in combin...

The technique that employs flash freezing and environmental scanning electron microscopy (ESEM) was utilised for detailed investigation of the fouling materials in a membrane bioreactor (MBR). The method involves the flash freezing of a wet sample in liquid nitrogen for 10s to preserve its structure for direct ESEM observation with a high image resolution. ESEM images show that the sludge cake ...

A method to examine the diffusible state and the sizes of major cytoplasmic proteins in a living cell is described. Small (40-300 microns) commercially available gel filtration beads of a broad range of Mr exclusion limits were microsurgically implanted into the cytoplasm of oocytes of the frog, Xenopus laevis, usually after metabolic labeling of oocyte proteins with [35S]methionine. After equi...