Longissimus dorsi muscle (LD) proteomics provides a novel opportunity to reveal the molecular mechanism behind intramuscular fat deposition. Unfortunately, the vast amounts of lipids and nucleic acids in this tissue hampered LD proteomics analysis. Trichloroacetic acid (TCA)/acetone precipitation is a widely used method to remove contaminants from protein samples. However, the high speed centri...

We encountered a 60-year-old woman with remarkably elevated thyroid-stimulating hormone (TSH) level as measured by electrochemiluminescent immunoassay (ECLIA), but with no specific symptoms, and with normal levels of free T3 and free T4. We performed the following investigations: polyethylene glycol (PEG) precipitation test, human antimouse IgG antibody (HAMA) interference test, and 3 additiona...

The alkaline phosphatase of leukocytes from normal subjects, patients with chronic granulocytic leukemia, and patients with reactive granulocytosis have been characterized by means of agarose column chromatography, sucrose density ultracentrifugation, starch gel electrophoresis, Michaelis constant for p-nitrophenyl phosphate, heat inactivation, inhibition by L-phenylalanine, Ouchterlony double ...

The solubility of the homogeneous, nonenzymatic structural protein isolated and purified from bovine heart mitochondria when incubated with the murine toxin of Pasteurella pesfis at pH 11.0 increases as the concentration of the toxin is increased. This indicates that a protein-protein interaction occurs between the monomeric units of the structural protein and the toxin. This interaction result...

Extraction of DNA from old skeletal material is of great importance in the identification of human remains, but is particularly difficult because the methods currently employed, especially those using phenol/chloroform, are not always satisfactory. A simple technique based on the removal of non-nucleic acid material by salting out (precipitation) with saturated sodium acetate is described; the ...

A rapid, simplified method for production and purification of tetanus toxin from bacterial extracts was described. The extracts were prepared by stirring young cells (ca. 45-h culture) of Clostridium tetani in 1 M NaCl-0.1 M sodium citrate, pH 7.5, overnight at 0 to 4 degrees C. The toxin was purified by a combination of (i) ammonium sulfate fractionation (0 to 40% saturation), (ii) ultracentri...

Batch hydrolysis of olive oil was performed by Candida rugosa lipase immobilized on Amberlite IRC-50 and Al2O3. These two supports were selected out of 16 carriers: inorganic materials (sand, silica gel, infusorial earth, Al2O3), inorganic salts (CaCO3, CaSO4), ion-exchange resins (Amberlite IRC-50 and IR-4B, Dowex 2X8), a natural resin (colophony), a natural biopolymer (sodium alginate), synth...

The solubility of the homogeneous, nonenzymatic structural protein isolated and purified from bovine heart mitochondria when incubated with the murine toxin of Pasteurella pesfis at pH 11.0 increases as the concentration of the toxin is increased. This indicates that a protein-protein interaction occurs between the monomeric units of the structural protein and the toxin. This interaction result...

A simple technique for seeking natural predators to mosquito larvae was developed. In the present study, adult backswimmers, a natural predator to mosquito larvae, were used as a model. The adult predators were fed with larvae and pupae of Aedes aegypti and tested for the presence of antigens of the mosquito larvae at 1, 2, 4, 6 and 24 hr thereafter. The test was performed using an immunologica...

The localization of individually specific antigenic determinants on the monoclonal gammaM Mö was studied in gel diffusion experiments with 19S gammaM Mö and its 7S subunit. Various types of such determinants were delineated. Some were demonstrable both on the intact macroglobulin molecule and on the subunit. The latter was often unable to precipitate with the corresponding antibody, but combine...