Physical properties are crucial to gelatin utilization and the physical properties are determined by structure. Therefore, it is important to investigate the nanostructure and physical properties of gelatin over the full range of concentrations which are widely applied in research and industry. Nanostructure of gelatin can be investigated by atomic force microscopy (AFM). However, it is hard to...

We have used fluorescein isosthiocyanate-conjugated gelatin (FITC-gelatin) (1 mg/ml) to localize cell surface fibronectin in unfixed live cells in cultures. FITC-gelatin stains the fibronectin matrix on primary cultures of rat and chick embryo fibroblasts as well as untransformed, established cell lines. In live cultured cells, fibronectin in many areas of the extracellular matrix is inaccessib...

Due to the many beneficial properties combined from both poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and liposomes, lipid-PLGA hybrid NPs have been intensively studied as cancer drug delivery systems, bio-imaging agent carriers, as well as antigen delivery vehicles. However, the impact of lipid composition on the performance of lipid-PLGA hybrid NPs as a delivery system has not bee...

Electrospinning has been recognized as an efficient technique for the fabrication of polymernanofibers. Recently, various polymers have successfully been electrospun into ultrafine fibers.Electrospinning is an extremely promising method for the preparation of tissue engineering scaffolds.In this study, nanofibers gelatin was electrospun at 20% v/v optimized content. To produce...

The effects of zinc oxide nanorod on the barrier, sorption isotherm and antibacterial properties of tapioca starch/bovine gelatin Bionanocomposite films were investigated. The nanorod-rich ZnO (ZnO-N) was homogenized by sonication and incorporated into tapioca starch/bovine gelatin solutions at different concentrations (e.g. 0.5, 2, and 3.5% w/w dried solid). Incorporation of 3.5% of nanopartic...

Low molecular weight chitosan (LMWC) is a promising polymer for surface modification of nanoparticles (NPs), which can impart both stealth effect and electrostatic interaction with cells at mildly acidic pH of tumors. We previously produced LMWC-coated NPs via covalent conjugation to poly(lactic-co-glycolic) acid (PLGA-LMWC NPs). However, this method had several weaknesses including inefficienc...

A PEG-based, folate mediated, active tumor targeting drug delivery system using DOX-hyd-PEG-FA nanoparticles (NPs) were prepared. DOX-hyd-PEG-FA NPs showed a significantly faster DOX release in pH 5.0 medium than in pH 7.4 medium. Compared with DOX-hyd-PEG NPs, DOX-hyd-PEG-FA NPs increased the intracellular accumulation of DOX and showed a DOX translocation from lysosomes to nucleus. The cytoto...

1. When solid blocks of isoelectric gelatin are placed in cold distilled water or dilute buffer of pH 4.7, only those of a gelatin content of more than 10 per cent swell, while those of a lower gelatin content not only do not swell but actually lose water. 2. The final quantity of water lost by blocks of dilute gelatin is the same whether the block is immersed in a large volume of water or whet...

Following preincubation of aprotinin with gelatin, the inhibitory effect on the trypsin activity was enhanced under the same condition as in the case of enhanced antigen-antibody reaction of aprotinin in gel in the presence of gelatin. In the immunochemical titration of aprotinin, gelatin treatment decreased the value of the equivalence point of aprotinin. Immunoprecipitation of aprotinin prein...

CONTENTS PAGE I. Introductory 147 II. Materials and methods ..148 III. The swelling of gelatin in water, hydrochloric acid and caustic soda . .148 IV. The state of gelatin dissolved by acid or alkali in the cold 153 V. The basic and acidic properties of gelatin . .155 VI. The behaviour of swollen gels in an atmosphere of saturated water vapour . .156 VII. Theory of gel structure deduced from a ...