In Escherichia coli, the synthesis of the flagellar organelle is sensitive to catabolite repression. Synthesis requires the presence of the cyclic adenosine monophosphate receptor protein (Crp) and 3',5'-cyclic adenosine monophosphate (cAMP); i.e., mutants that lack Crp or adenylcyclase (Cya) synthesize no flagella. We isolated and characterized a series of mutants (cfs) that restored flagella-...

Amino acid control of ribonucleic acid (RNA) synthesis in bacteria is known to be governed genetically by the rel locus. We investigated whether the rel gene of the host would also exert its effect on the regulation of phage-specific RNA synthesis in T4 phage-infected Escherichia coli cells. Since T-even phage infection completely shuts off host macromolecular synthesis, phage RNA synthesis cou...

Abstract Maize is one of the three major crops worldwide based on its yield and quality. Starch crucial to both quality maize as it accounts more than 60% seed weight, structure influences crop. synthase I (SSI) contributes majority starch activity in endosperm. An in-depth understanding synthesis regulatory mechanism would provide opportunities for improving maize. In this study, ZmPLATZ2 , a ...

Testing the deluge of hypotheses flowing from genomics and systems biology demands accurate and costeffective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of "construction" oligonucleotides and tagged complementary "selection" oligonucleotides are synthesized on photo-programmable microfluidic chips, released, amplifie...

Gene Selection is one class of most used data analysis algorithms on microarray datasets. The goal of gene selection algorithms is to filter out a small set of informative genes that best explains experimental variations. Traditional gene selection algorithms are mostly single-gene based. Some discriminative scores are calculated and sorted for each gene. Top ranked genes are then selected as i...

The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potentially lead to breakthroughs in the development of accurate, low-cost and high-...

We recently reported that hairpin (or stem-loop) priming is better-suited than polyA tailing to generate cDNA for plant microRNA qPCR. One major limitation of this method is the need to perform individual cDNA synthesis reactions for the reference gene and test miRNAs. Here, we report a novel fusion primer that allows multiplexed hairpin cDNA synthesis of the most-commonly used reference gene, ...

on expression of the Xrex+ gene, since little inhibition was observed following infection by a Xrex mutant or by a hybrid bacteriophage Xii44 which lacks a functional rex gene. For promotion of the synthesis of rex product, cII+ and N+ genes are required and can act trans, whereas cY+, also required, must be cis to a rex+ gene. These genes presumably play a role in the transcription of the cd-r...

Phenotypic reversion of six independent Saccharomyces cerevisiae cho1 mutants was shown to be due predominantly to mutation of an unlinked gene, eam1. The eam1 gene was located very close to ino1 on chromosome X by meiotic tetrad analysis. Recessive eam1 mutations did not correct the primary cho1 defect in phosphatidylserine synthesis but made endogenous ethanolamine available for sustained nit...