Different genetic markers were used to analyze 22 Mycobacterium bovis strains isolated from cattle in Sardinia and one human isolate. IS6110 DNA fingerprinting differentiated the strains into six patterns, whereas with enterobacterial repetitive consensus sequence primers produced seven clusters. PCR ribotyping followed by digestion with HaeIII and PvuII produced five and seven patterns, respec...

Breeding progress in black raspberry (Rubus occidentalis L.) has been limited by a lack of genetic diversity in elite germplasm. Black raspberry cultivars have been noted for showing very few phenotypic differences and seedlings from crosses between cultivars for a lack of segregation for important traits. Despite these challenges, little molecular work has been done to explore genetic diversit...

Conservation genetics focuses on the effects of contemporary genetic structuring on long-term survival of a species. It helps wildlife managers protect biodiversity by identifying a series of conservation units, which include species, evolutionarily significant units (ESUs), management units (MUs), action units (AUs), and family nets (FNs). Although mitochondrial DNA (mtDNA) evolves 5-10 times ...

various molecular techniques are available to make differences and to study the genetic divergences among species and populations, of which the most efficient ones are pcr-based markers. one of these methods, molecular fingerprinting, enables the distinction of closely related populations. issr-primers are universal primers that can be used for such studies. the utility of these primers was tes...

DNA is a unique piece of genetic material within biological organisms. Nowadays, molecular techniques can be used to generate DNA fingerprinting in many species of animals. It is used in a variety of applications in veterinary medicine such as parentage testing, wildlife investigation and individual and sex identification. The samples can be collected from any biological samples such as blood, ...

The purpose of this article is to review the status of DNA assays used for the detection, identification, and individualization of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, and Brucella abortus. These select agent microorganisms are historically significant as they have either been used or experimented with as a bioweapon or as a terrorist agent and are t...

We have developed an approach for identifying overlapping cosmid clones by exploiting the high density of repetitive sequences in complex genomes. Individual clones are fingerprinted, using a combination of restriction enzyme digestions followed by hybridization with selected classes of repetitive sequences. This "repeat fingerprinting" technique allows small regions of clone overlap (10-20%) t...

introduction: recently, the incidence of fungal infections dramatically increased with an appearance of many novel species due to different criteria. therefore, several molecular methods have been established for identification of these agents which cause human and animal disorder based on genomic dna. among these methods, rapd-pcr technique is a powerful discriminative method based on amplifie...

garlic (allium sativum .l) has been and is clonally propagated, because it does not produce seed under standard conditions. knowledge of plant recourses genetic diversity can be a first and most important step in achieving breeding program purposes. because of its high accuracy and reproducibility among different genetic diversity assessment techniques amplified fragment length polymorphism (af...

Preimplantation genetic diagnosis (PGD) has experienced a considerable technical evolution since its first application in the early 1990s. The technology for single-cell genetic analysis has reached an extremely high level of accuracy and enabled the possibility of performing multiple diagnoses from one cell. Diagnosis of a monogenic disease can now be combined with aneuploidy screening, human ...