The Clustered Regularly Interspaced Short Palindromic Repeats associated Cas9/sgRNA system is a novel targeted genome-editing technique derived from bacterial immune system. It is an inexpensive, easy, most user friendly and rapidly adopted genome editing tool transforming to revolutionary paradigm. This technique enables precise genomic modifications in many different organisms and tissues. Ca...

Genome-editing technology, although a robust tool for genetic engineering, is creating indistinct regulatory boundaries between naturally occurring and modified organisms. However, researchers must act with caution in research and development to avoid misleading society. Furthermore, appropriate regulations should be proactively discussed and established for handling genome-editing technology.

The CRISPR-Cas9 system (clustered regularly interspaced short palindromic repeats with associated Cas9 protein) has been used to generate targeted changes for direct modification of endogenous genes in an increasing number of plant species; but development of plant genome editing has not yet fully considered potential off-target mismatches that may lead to unintended changes within the genome. ...

MOTIVATION Insertional RNA editing renders gene prediction very difficult compared to organisms without such RNA editing. A case in point is the mitochondrial genome of Physarum polycephalum in which only about one-third of the number of genes that are to be expected given its length are annotated. Thus, gene prediction methods that explicitly take into account insertional editing are needed fo...

Genome editing is a generic term for new methods enabling targeted modifications to the genetic material (genome) of a cell. The CRISPR/Cas9 method in particular holds great promise for new application methods. The German Federal Institute for Risk Assessment (BfR) observes these scientific developments in the interest of consumer health protection. Here the BfR answers the most important quest...

High efficiency, homology-directed genome editing in C. elegans using CRISPR/Cas9 ribonucleoprotein complexes Alexandre Paix, Andrew Folkmann, Dominique Rasoloson, Geraldine Seydoux HHMI and Dept of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore MD 21205, USA Running title: Genome editing in C. elegans using CRISPR/Cas9 RNP complexes