background: hydatidosis or cystic hydatid disease is one of the most important diseases in human and animals. identification of strains is important for improvement of control and prevention of dis-ease. the aim of this study was to determine the strains isolated from human and domestic animals in ilam province, iran, using pcr-rflp method. methods: respectively, 30 and 4 animal and human hydat...

Material and methods Eight genotypes of 4 most common SNPs localised in GPX1, GPX4, TXNRD2 and SEP15 were selected. Genotyping was performed in 93 affected and 186 unaffected matched BRCA1 carriers as well as on pairs matched 1:1 consisting of 108 breast, 50 ovarian and 105 prostate consecutive cancer patients and healthy controls. The following techniques for laboratory analyses have been appl...

The application of genotyping to clinical isolates of Cryptosporidium has increased significantly our knowledge and understanding of the distribution and epidemiology of this parasite. However, some methods can be laborious and demand specialist technical expertise. PCR-restriction fragment length polymorphism (RFLP) techniques represent a more rapid and simple method of genotyping to support e...

Such difficult analyses as disease association studies, which aim at mappping genetic variants underlying complex human diseases, rely on high-throughput genotyping techniques. However, a shortcoming of these techniques is the generation of missing calls. Computational inference of missing data represents a challenging alternative to genotyping again the missing regions. In this paper, we prese...

â  â  background hepatitis c virus (hcv) infection is responsible for considerable morbidity and mortality worldwide. the hcv genotype has a geographic distribution and an important role in clinical and histological outcomes. this study determined hcv genotypes and their related risk factors among patients from khuzestan province, southwest iran. methods in a cross-sectional study, 223 patients...

Genotyping methods for Plasmodium falciparum drug efficacy trials have not been standardized and may fail to accurately distinguish recrudescence from new infection, especially in high transmission areas where polyclonal infections are common. We developed a simple method for genotyping using previously identified microsatellites and capillary electrophoresis, validated this method using mixtur...

hepatitis c is a major cause of liver related morbidity and mortality worldwide and represents a major public health problem. depending on genomic organization, the virus is divided into six genotypes and a number of subtypes. different genotypes are seen in different parts of the world. genotype one is difficult to treat, while genotypes 2 and 3 are easy to treat. therefore, identification of ...

Although recent developments in DNA sequencing have allowed for great leaps in both the quality and quantity of genome assembly projects, de novo assemblies still lack the efficiency and accuracy required for studying genetic variation of individuals. Thus, efficient and accurate methods for calling and genotyping genetic variants are fundamental to studying the genomes of individuals. We study...

Previous genetic studies of orangutans (Pongo spp.) have relied mainly upon mitochondrial DNA or microsatellite short tandem repeats (STR) for genomic genotyping analysis. Scientists yet to take advantage the closeness great apes humans analysis by using advanced techniques available human genotyping. To genotype at Tanjung Puting National Park, we developed a novel combination methyl-based mag...

multilocus variable number tandem repeat (vntr) analysis (mlva) is a new typing method with several advantages compared to other methods. dissemination of shigella is highly significant in developing countries. whilst shigella is becoming increasingly important as an etiologic agent of pediatric shigellosis in iran, little is known about the genetic diversity of the local strains. therefore, th...