Methotrexate (MTX) is widely used for the treatment of childhood acute lymphoblastic leukemia (ALL). The accumulation of MTX and its active metabolites, methotrexate polyglutamates (MTXPG), in ALL cells is an important determinant of its antileukemic effects. We studied 194 of 356 patients enrolled on St. Jude Total XV protocol for newly diagnosed ALL with the goal of characterizing the intrace...

Artificial nucleases containing Rev-coupled metal chelates based on combinations of the transition metals Fe(2+), Co(2+), Ni(2+), and Cu(2+) and the chelators DOTA, DTPA, EDTA, NTA, tripeptide GGH, and tetrapeptide KGHK have been tested for DNA nuclease activity. Originally designed to target reactive transition metal chelates (M-chelates) to the HIV-1 Rev response element mRNA, attachment to t...

BACKGROUND While initially sensitive to heat shock, the bovine embryo gains thermal resistance as it progresses through development so that physiological heat shock has little effect on development to the blastocyst stage by Day 5 after insemination. Here, experiments using 3' tag digital gene expression (3'DGE) and real-time PCR were conducted to determine changes in the transcriptome of morul...

OBJECTIVE The aim of this study was to examine the differences in the health related quality of life and the self care and social function in daily life of children with different disabilities. METHODS One hundred and two children with physical, emotional and cognitive disabilities (cerebral palsy, mental retardation, and hearing loss) and 28 children age matched as a control group were inclu...

Catalytic metallodrugs were used to oxidatively cleave HIV-1 Rev Response Element RNA (RRE RNA), and the mechanisms of RNA cleavage were studied using a combination of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), fluorescence spectroscopy, and gel electrophoresis. The metallodrugs, which contained combinations of the transition metals Fe2+, Co2+, ...

UNLABELLED It is known that cervical cancer develop from precancerous intraepithelial neoplasia (CIN) which is characterized by series of genetic abnormalities. The progression of CIN to cervical carcinoma has been associated especially with the genomic integration of oncogenic human papilloma virus (HPV) and gain of the human telomerase RNA gene hTERC (3q26) and MYC (8q24). In this study, cyto...

PATRICIA A. BARFORD, JOHN A. BLAIR and FREDERICK J. STAFF Department of Chemistry, University of Aston in Birmingham, Gosta Greem, Birmingham B4 7ET, U.K. It has been frequently claimed that polyglutamate forms of folates are found in animals (Noronha & Silverman, 1962; Shin et al., 1972; Brown et al., 1974) on the basis of column chromatography of these folates and chromatography of 'authentic...

The one-loop process eγ → eH , for intermediate Higgs masses is considered. Exact cross sections for unpolarized and longitudinally polarized beams are computed and found to be more than two orders of magnitude larger than the rates for the crossed process e+e− → Hγ , in the energy range√s = (0.5÷2) TeV. We show that, apart from being competitive with the γγ → H process for testing the one-loop...

In the field of structural health monitoring or machine condition monitoring, the activation of nonlinear dynamic behavior complicates the procedure of damage or fault detection. Blind source separation (BSS) techniques are known as efficient methods for damage diagnosis. However, most of BSS techniques repose on the assumption of the linearity of the system and the need of many sensors. This a...