The yeast prions [PSI+] and [URE3] are folded in-register parallel β-sheet amyloids of Sup35p and Ure2p, respectively. In a screen for antiprion systems curing [PSI+] without protein overproduction, we detected Siw14p as an antiprion element. An array of genetic tests confirmed that many variants of [PSI+] arising in the absence of Siw14p are cured by restoring normal levels of the protein. Siw...

Our earlier studies with the pgsA mutant of Synechocystis PCC6803 demonstrated the important role of phosphatidylglycerol (PG) in PSII dimer formation and in electron transport between the primary and secondary electron-accepting plastoquinones of PSII. Using a long-term depletion of PG from pgsA mutant cells, we could induce a decrease not only in PSII but also in PSI activity. Simultaneously ...

Peroxiredoxins (Prxs) are ubiquitous antioxidants that protect cells against oxidative stress. We show that the yeast Tsa1/Tsa2 Prxs colocalize to ribosomes and function to protect the Sup35 translation termination factor against oxidative stress-induced formation of its heritable [PSI(+)] prion conformation. In a tsa1 tsa2 [psi(-)] [PIN(+)] strain, the frequency of [PSI(+)] de novo formation i...

Photosystem I (PSI) from Chroococcidiopsis thermalis PCC 7203 grown under far-red light (FRL; >725 nm) contains both chlorophyll a and a small proportion of chlorophyll f. Here, we investigated excitation energy transfer and charge separation using this FRL-grown form of PSI (FRL-PSI). We compared femtosecond transient visible absorption changes of normal, white-light (WL)-grown PSI (WL-PSI) wi...

نم أشنت .ةردان ةديمح ماروأ يه )ةيفيللا( ةيطابرلا مارولأا عباط تاذ يهو .مسلجا ءاحنأ عيمج يف ةيلضعلا لكايهلا لدعم عافترا ىلإ ىدأ امم ، ةرواجلما هيللمحا ةجسنلأل يحاشترا ةردنل ًارظن و .يحارلجا لاصئتسلاا دعب مرولا عوجر ةبسنو نع غلابلإاب انمق اننإف ، ةبقرلاو سأرلا ةقطنم يف مارولأا هذه يف اهجلاعو قنعلا ىلعأ يف يطابرلا مرولاب اهصيخشت تم ةلاح جلاعل هرفوتلما تارايلخاو قرطلا لوح يعولا ةدايزل ،انافشتسم .ةبق...

The yeast cytoplasmically inherited genetic determinant [PSI+] is presumed to be a manifestation of the prion-like properties of the Sup35 protein (Sup35p). Here, cell-free conversion of Sup35p from [psi-] cells (Sup35ppsi-) to the prion-like [PSI+]-specific form (Sup35pPSI+) was observed. The conversion reaction could be repeated for several consecutive cycles, thus modeling in vitro continuou...

Systematic spectral analysis of Phase Shifting Interferometry (PSI) algorithms was first proposed in 1990 by Freischlad and Koliopoulos (F&K). This analysis was proposed with the intention that "in a glance" the main properties of the PSI algorithms would be highlighted. However a major drawback of the F&K spectral analysis is that it changes when the PSI algorithm is rotated or its reference s...

We have been reporting several new techniques of analysis and synthesis applied to Phase Shifting Interferometry (PSI). These works are based upon the Frequency Transfer Function (FTF) and how this new tool of analysis and synthesis in PSI may be applied to obtain very general results, among them; rotational invariant spectrum; complex PSI algorithms synthesis based on simpler first and second ...

This paper constructs fully parallel NN hardware architecture, FPGA has been used to reduce neuron hardware by design the activation function inside the neuron without using lookup table as in most researches, to perform an efficient NN. It consist of two main parts; the first part covers network training using MATLAB program, the second part represents the hardware implementation of the traine...