Differential scanning calorimetry (DSC) was used to determine the thermal events associated with freezing and melting of the cysts of the potato-cyst nematode Globodera rostochiensis. There were no thermal events during the cooling of dry cysts from 5 to -60 °C and warming back to 5 °C, indicating the absence of water freezing in the dry cysts. During heating of dry cysts from 5 to 80 &...

Globodem roswch!ens!s and G. pall!da harched (l-day-old in poraro roor diffusare) second srage juveniles 02) conrained 29.2 % and 27.2 % lipid (of dry weighr), respectively. Globodera roslOch!ens!s J2 lipid consisred of 72.8 % neutral lipid, 11.3 % free fatty acids and 15.9% non-acetic phospholipid. The neutral lipid fraction comprised 95.5 % triacylglycerides, 1.8 % diacylglycerides, 2.3 % mon...

The tobacco cyst nematode (Globodera tabacum solanacearum) continues to pose a serious threat to flue-cured tobacco production in Virginia and nearby states. Soils were sampled from five uninfested and two infested flue-cured tobacco-producing locations. Twenty-three edaphic factors were characterized to determine if any were correlated with G. t. solanacearum reproduction. Comparisons were als...

The essential and ubiquitous enzyme fructose bisphosphate aldolase (FBPA) has been a good target for controlling the various types of infections caused by pathogens and parasites. The parasitic infections of nematodes are the major concern of scientific community, leading to biochemical characterization of this enzyme. In this work we have developed a small dataset of all types of FBPA sequence...

To understand the machinery underlying a tomato cultivar harboring the Hero A gene against cyst nematode using microarrays, we first analyzed tomato gene expression in response to potato cyst nematode (PCN; Globodera rostochiensis) during the early incompatible and compatible interactions at 3 and 7 days post-inoculation (dpi). Transcript levels of the phenylalanine ammonia lyase (PAL) and Myb-...

The potato rot nematode, Ditylenchus destructor, is a very destructive nematode pest on many agriculturally important crops worldwide, but the molecular characterization of its parasitism of plant has been limited. The effectors involved in nematode parasitism of plant for several sedentary endo-parasitic nematodes such as Heterodera glycines, Globodera rostochiensis and Meloidogyne incognita h...