1. We have correlated measurements of colloid osmotic pressure with protein concentrations and albumin/globulin ratios. Plasma from normal rats of different genetic strains was used. 2. The Landis and Pappenheimer equation does not accurately predict colloid osmotic pressure properties of the normal rat plasma used, which had an albumin/globulin ratio of 0.74. Over the range 2-2-12-2 g of prote...

The effect of woundingand formation of regenerating wound tissueon the level of the serum proteins was studied in rats on a protein-free diet. It was found that the level of the globulin component having the third greatest electrophoretic mobility, probablypart of the a,-globulin fraction, roseabout 60% within 3 days after wounding and did not begin to return to normal until about 10 days later...

An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acet...

Purification of the activity of the fourth component of human complement resulted in the isolation of a highly homogeneous serum protein. Since this protein has not been recorded previously it was called beta(1E)-globulin on the basis of its immunoelectrophoretic behavior. C'4 activity and beta(1E)-globulin were found to have highly similar, if not identical physicochemical characteristics. Mor...

An attempt is made to describe the nature of the chemical interactions participating in blood clotting. In the laboratory, thrombin can be derived directly from prothrombin without the physiologic activators calcium, thromboplastin, Ac-globulin, and platelet derivatives. The newly recognized clotting factor, Ac-globulin, is essential for normal physiologic hemostasis. Plasma Ac-globulin concent...

An attempt is made to describe the nature of the chemical interactions participating in blood clotting. In the laboratory, thrombin can be derived directly from prothrombin without the physiologic activators calcium, thromboplastin, Ac-globulin, and platelet derivatives. The newly recognized clotting factor, Ac-globulin, is essential for normal physiologic hemostasis. Plasma Ac-globulin concent...

One of us (LWS) bad occasion to treat a young white man with gamma globulin for prophylaxis after exposure to infectious hepatitis. He was given two injections, 10 cc. each, of human Poliomyelitis Immune Globulin, one week apart. It was observed that shortly after the second injection, his severe pustular and indurated acne improved considerably. This observation led to the present study, of wh...

Hypophysectomy is known to cause complete suppression of the hepatic synthesis az,-globulin. The effect of hypophysectomy on the synthesis of azu-globulin can be reversed by multiple hormone treatment. The role of pituitary growth hormone in the multihormonal regulation of aZ,-globulin in rat liver was examined in the hypophysectomized male rats with and without growth hormone supplementation. ...

S-sulfonated gamma globulin (GGS), newly developed as a safe drug for intravenous use, was studied for its protective effects against some experimental infections in mice. Gamma globulin showed a good protective activity against infections due to Streptococcus pneumoniae and Escherichia coli and was moderately active against infections due to Staphylococcus aureus and Pseudomonas aeruginosa. In...

1863 liter three years before admission. Instead of providing immediate immune globulin replacement on admission, his clinicians remeasured the serum IgG five days later (130 mg per deciliter), and immune globulin replacement was given seven days after admission. The patient died on the 12th hospital day. There is an extensive body of literature supporting the value of intravenous immune globul...