Brachypodium distachyon, a small annual grass with seed storage globulins as primary protein reserves was used in our study to analyse the toxic nature of non-prolamin seed storage proteins related to celiac disease. The main storage proteins of B. distachyon are the 7S globulin type proteins and the 11S, 12S seed storage globulins similar to oat and rice. Immunoblot analyses using serum sample...

A technique for measuring the alkaline phosphatase activity of serum protein fractions separated by electrophoresis on cellulose acetate strips is described. Alkaline phosphatase activity in alpha 2, alpha 1, and beta globulins and in albumin is present in varying proportions in normal and pathological sera. In normal sera peak activity is in the alpha 2 globulins and beta globulins have a litt...

BACKGROUND Retired racing Greyhounds are becoming common as pets. Because of their unique physiology, results of routine laboratory tests are frequently outside the reference interval for dogs. Compared with other breeds, Greyhounds have low serum protein concentrations, but the concentrations of different serum protein fractions have not been reported. OBJECTIVES Our objectives were to evalu...

Malignant neoplasia is thought to develop through multifactorial gene stimulations which leads to marked variations in plasma proteins. The study aims at the quantification of serum albumin and different globulin fractions in 28 multiple myeloma and 25 other hematological cancer and compare the alterations with that of 40 age and sex matched normal subjects. Serum albumin and total proteins wer...

A 17 year old girl with active Crohn’s colitis developed idiopathic thrombocytopenic purpura that was managed with intravenous immune globulins and cyclosporin A. The possible association between Crohn’s disease and immune thrombocytopenia is explored. (Postgrad Med J 2000;76:299–300)

Bence-Jones proteins are commonly found in patients with multiple myeloma and have been defined classically' as abnormal urinary proteins that have characteristic thermosolubility properties. Structural studies2 have shown that BenceJones proteins are similar or identical to the light (L) polypeptide chains isolated from the myeloma globulin of the same patient. Taken in conjunction with the re...

A 17 year old girl with active Crohn's colitis developed idiopathic thrombocytopenic purpura that was managed with intravenous immune globulins and cyclosporin A. The possible association between Crohn's disease and immune thrombocytopenia is explored.

Behrens in 1932 (1) developed a method for isolating cellular nuclei. Lyophilized tissue was finely ground and the nuclei were isolated from other cellular debris by repeated centrifugation in organic solvents of suitable densities. The greater density of the nuclei makes this procedure possible. The method has been employed by various workers (2-5). Dounce et al. (4) reported that approximatel...

Conventionally prepared immune serum globulin frequently produces severe side effects when administered intravenously. A modified preparation in which 4 to 5 interchain disulfide bonds have been reduced and alkylated has been made for intravenous use. However, reduction and alkylation may affect Fc-mediated functions of immunoglobulin G, particularly its ability to fix complement by the classic...

The aim of this study was to evaluate the changes of serum total proteins, albumin, α1-, α2-, β1-, β2 and γ -globulins in growing foals during the first month of life. Nine clinically healthy foals (five females and four males, mean body weight 39.00 ± 3.50 kg) were enrolled in the study. From each animal, blood samples were collected by jugular venipuncture every 3 days during the first month ...