The present study was designed to determine the effects of the essential oil of Laurusnobilis L. (Lauraceae) and its three main components on α-glucosidase and reactive oxygenspecies scavenging activity. The chemical composition of the essential oil from Laurus nobilisL. leaves was analyzed by GC/GC-MS and resulted in the identification of 29 compounds,representing 99.18% of the total oil. 1,8-...

  Background: Hypoglycemic effect of Juglans regia leaves was reported in other previous researches.   Objective: To investigate the mechanism of hypoglycemic action of Julans regia Leaves Methanolic Extract (JRLME), we designed the following study.   Methods: Male wistar rats were classified in five groups, each of six, including alloxan-induced diabetic rats treated with JRLME at doses of 250...

this study focused on use of bacterial extracellular enzyme activities as biochemical parameters, along with the microbiological and physicochemical characteristics, in a comprehensive assessment of water quality of four lake ecosystems in the province of vojvodina (northern serbia): provala, ludas, zobnatica and palic. water samples were collected in june and october, 2008. for assessment of ...

This study was designed to investigate the inhibitory effects of Polyopes lancifolia extract (PLE) on α-glucosidase activity, α-amylase activitiy, and postprandial hyperglycemia in streptozotocin (STZ)-induced diabetic mice. The results of this study revealed a marked inhibitory effect of PLE on α-glucosidase and α-amylase activities. The IC50s of PLE against α-glucosidase and α-amylase were 0....

Previous studies of alpha-glucosidase inhibitors derived from thalidomide revealed that 4,5,6,7-tetrachloro-N-alkylphthalimide derivatives are superior lead compounds. Structure-activity relationship studies indicated that a hydrophobic group at the N(2) position is mandatory for potent activity. Accordingly, we have designed and synthesized some 4,5,6,7-tetrachloro-N-cycloalkylphthalimide and ...

Three new eremophilane sesquiterpenes (1-3) were isolated from the mangrove endophytic fungus Xylaria sp. BL321 together with 07H239-A (4), a known analogue of the new compounds. The structures of these compounds were elucidated by analysis of their MS, 1D and 2D NMR spectroscopic data. Compound 4 showed activation activity on α-glucosidase at 0.15 μM (146%), and then, 4 gradually produced inhi...

Glycogen autophagy in newborn rat hepatocytes was studied by using enzyme determinations and electron microscopy. Cyclic AMP induced glycogen autophagy in these cells. Glycogen-hydrolyzing acid glucosidase activity increased whereas acid mannose 6-phosphatase activity decreased in the liver of these animals. Parenteral glucose, which prevents postnatal glucagon secretion and tissue cyclic AMP e...

This study investigated the antioxidant activity of methanol (MeOH) and water extracts from roots of Cirsium japonicumin vitro. MeOH extract showed a stronger free radical scavenging activity than water extract. However, both of extracts showed a concentration dependent hydroxyl radical scavenging activity, reducing power and metal chelating ability. MeOH extract had greater phenolic and flavon...

I applied the fluorescein diacetate (FDA) hydrolysis technique as a rapid and sensitive estimator of total esterase activity in stream sediment biofilms. I investigated the effects of temperature, pH and incubation time and optimized the assay for low blanks and high fluorescein extraction. The FDA procedure was precise (c.v. = 4.15%) and could detect 10 nM fluorescein. Spatial patterns of este...