The effects of storage conditions and cooking methods on chlorophyll, glucosinolate (GSL), sulforaphane content in broccoli florets were investigated this study. For the experiment, fresh stored for 2, 4, 6 days at 10, 0 °C with or without 1-methylcyclopropene (1-MCP) treatment. cooked 1, 3, 5, 7, 9 min under three methods, namely, steaming, microwaving, boiling. Results showed that contents al...

Glucosinolates are sulfur-rich secondary metabolites characteristic of the Brassicales order with important biological and economic roles in plant defense and human nutrition. Application of systems biology tools continues to identify genes involved in the biosynthesis of glucosinolates. Recent progress includes genes in all three phases of the pathway, i.e. side-chain elongation of precursor a...

Plant secondary metabolism comprises an enormous diversity in compounds and enzymes, and wide spectra of mechanisms of gene regulation and of transport of metabolites and enzymes. Genetic approaches using the model plant Arabidopsis thaliana have contributed importantly to recent progress in understanding glucosinolate biosynthesis and its intricate linkage with auxin homeostasis. Arabidopsis g...

Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called "mustard oil bomb" is disarmed by a glucosinolate sulfatase of a crucifer specialist insect, diamondback moth, Plutella xylostella ...