background: serum procalcitonin (pct) levels differ in patients with bacterial or fungal infections and are significantly elevated in patients with gram-negative bacteremia. we evaluated the diagnostic accuracy of different inflammatory markers to discriminate sepsis caused by different pathogens. materials and methods: we included 328 episodes of bacteremia from 292 patients with sepsis and 31...

Sequence analysis of the Helicobacter pylori major sigma factor (RpoD) shows that it is highly divergent, which may be related to the marked diversity of the H. pylori chromosome. Furthermore, the rate of divergence of RpoD among other gram-negative bacteria is much greater than that among gram-positive bacteria. This suggests that RpoD from gram-negative bacteria is functionally less constrain...

The insertion and the permanence of central venous catheters (CVC) represent potential sources of infection contracted in hospital. The evaluation of the risk of CVC-associated infections was evaluated in a retrospective study during the period 2007-2010 in a Hospital of Central Italy. A total of 514 CVC were collected and examined by microbiological techniques and, among the examined patients,...

The Danish microbiologist Hans Christian Gram developed a staining technique (1884) that classifies most bacteria into two large groups that are referred to eponymously, as Gram-positive and Gram-negative bacteria (1). His stain is still used in medical microbiology labs today, and because of its explanatory power, this classification is the most commonly used descriptor of a particular bacteri...

Media containing the fluorogenic compound 8-anilino-1-naphthalene sulfonic acid may be used to discriminate between gram-positive and gram-negative bacteria and to differentiate between various species of bacteria. Fluorescent light emitted from colonies of gram-negative bacteria on 8-anilino-1-naphthalene sulfonic acid-containing agar was visually more intense than that on gram-positive bacter...

The Gram stain classifies most bacteria into one of two groups, Gram-negative or Gram-positive, based on the composition their cell walls [...]

the antimicrobial activity of thirty six novel dihydropyrimidine derivatives was evaluated against common pathogenic bacteria. significant antimicrobial activity (mic=32, 64 µg/ml) was observed. escherichia coli and pseudomonas aeruginosa as gram-negative bacteria and staphylococcus aureus as gram-positive bacteria were determined to be the most susceptible pathogens in this study. the highest ...

Objective. To examine whether Gram staining can influence the choice of antibiotic for the treatment of peritonsillar abscess. Methods. Between 2005 and 2009, a total of 57 cases of peritonsillar abscess were analyzed with regard to cultured bacteria and Gram staining. Results. Only aerobes were cultured in 16% of cases, and only anaerobes were cultured in 51% of cases. Mixed growth of aerobes ...

Background/objective: Neonatal septicemia is one of the major causes of mortality in newborns. The aim of this study is to evaluate the clinical manifestations and laboratory findings in positive blood culture in neonatal septicemia. Methods: In this retrospective study, we allocated 100 records positive blood culture of neonates suffering from septicemia. A questionnaire was completed for each...