The isolation of lignin is of great importance to understand its structural characteristics. A lithium chloride/dimethyl sulfoxide (LiCl/DMSO) solvent system has been developed for the dissolution of lignocellulose and for the isolation of lignin for this purpose. In this work, ball-milled wheat leaf (sheath included) was dissolved in the LiCl/DMSO solvent system and then regenerated in water. ...

The carboxyl ester lipase (CEL) gene is highly expressed in exocrine pancreas and expression of the human CEL gene is mediated by a strong tissue-specific enhancer, which is absolutely necessary for high-level expression. The mouse promoter, on the other hand, does not contain a corresponding enhancer element, but instead is totally dependent on another pancreas-specific element. This element i...

A sensitive and reliable LC-(ESI)MS/MS method was developed and validated for the simultaneous analysis of five common advanced glycation endproducts (AGEs) after enzymatic digestion in raw and roasted almonds. AGEs included carboxymethyl-lysine (CML), carboxyethyl-lysine (CEL), pyralline (Pyr), argpyrimidine (Arg-p), and pentosidine (Pento-s). This method allows accurate quantitation of free a...

We used co-cultures of porcine ovarian granulosa cells and mouse adrenocortical tumor cells (Y-1) to examine the kinetics of contact-dependent intercellular signal transfer and to assess the molecular mechanisms employed by this process. Exposure to follicle-stimulating hormone (FSH) caused cAMP-dependent protein kinase dissociation in granulosa cells and, with time, in Y-1 cells if, and only i...

The expression of mRNA for the activin type II receptors (ActRII and ActRIIB), follistatin, and the inhibin/activin subunits was investigated in the follicles of broiler breeder hens. Total RNA was isolated from individual granulosa and theca layers of the F1 through F5 follicles, a pool of the F6 and F7 follicles, the small yellow follicles, and from the combined granulosa and theca layers of ...

To investigate the effect of thyroid hormone on the proliferative activity and apoptosis of granulosa cells at the varying stages of follicular growth, porcine granulosa cells obtained from small (1-2 mm), medium (3-5 mm) and large (6-11 mm) follicles were cultured under a serum-free condition in the presence or absence of follicle stimulating hormone (FSH; 20 ng/ml), with or without triiodothy...

The Reverse Talmud rule for bankruptcy problems applies the Constrained Equal Awards rule (CEA rule) for ‘large’ amounts of the estate, while it applies the Constrained Equal Losses rule (CEL rule) for ‘small’ amounts of the estate. The CEA rule, respectively CEL rule, can be axiomatized using the Exemption and Exclusion properties respectively. There is no rule that satisfies both these two pr...

CEL-III is a hemolytic lectin isolated from Cucumaria echinata. Although recombinant CEL-III (rCEL-III) expressed in Escherichia coli showed very weak hemolytic activity compared with native protein, it was considerably enhanced by refolding in the presence of Ca(2+). This suggests that Ca(2+) supported correct folding of the carbohydrate-binding domains of rCEL-III, leading to effective bindin...