The aims of this study were to establish a random amplified polymorphic DNA (RAPD) fingerprint database of chloroplast DNA (cpDNA) from different cultivars of Cornus officinalis and to convert RAPD markers to sequence characterized amplified regions (SCAR) markers. A method of extraction was established that was suitable for obtaining cpDNA from samples rapidly dried in silicone; an RAPD finger...

Major soluble proteins of grapevine ripe berries were extracted from six different cultivars including non vinifera, with trichloroacetic acid acetone and resolved in two-dimensional electrophoresis (2-DE) gels. About three hundred spots were detected on the 2-DE map after colloidal blue staining. From 2-DE map of cv. Gamay mesocarp, 67 proteins were identified (p > 0.95) using matrix-assisted ...

Eutypa lata is a fungal pathogen causing severe dieback in vineyards worldwide. This fungus colonizes vines through pruning wounds, eventually causing a brown sectorial necrosis in wood as well as stunted vegetative growth. Several years may pass between infection and the expression of external symptoms, hindering the rapid evaluation of both grapevine cultivars susceptibility and E. lata varia...

As we reported earlier, Muscat Bailey A (MBA) wine has very low proanthocyanidin (PA) content compared with Cabernet Sauvignon (CS) and Merlot wines. Whereas PA concentration in CS was maintained at the maximum level during maceration, that in MBA reached a maximum approximately four days after maceration and drastically decreased thereafter. In this study, the model wine extraction profiles of...

BACKGROUND The phytohormone gibberellic acid (GA3) is widely used in the table grape industry to induce seedlessness in seeded varieties. However, there is a paucity of information concerning the mechanisms by which GAs induce seedlessness in grapes. METHODOLOGY/PRINCIPAL FINDINGS In an effort to systematically analyze the cause of this GA3-induced seed abortion, we conducted an in depth char...

A graft-transmissible disease displaying red veins, red blotches and total reddening of leaves in red-berried wine grape (Vitis vinifera L.) cultivars was observed in commercial vineyards. Next-generation sequencing technology was used to identify etiological agent(s) associated with this emerging disease, designated as grapevine redleaf disease (GRD). High quality RNA extracted from leaves of ...

Climate is a limiting factor in viticulture, as it defines favorable areas, grape cultivars, and agrotechnical activities. In the Sevastopol region, viticulture main promising agricultural branch. Using outputs of regional climate models from CORDEX project, projections agroclimatic conditions region for two future periods (2021–2045 2046–2070) under representative concentration pathways (RCP4....