Natural and anthropogenic pressures in inland waters induce molecular response mechanisms organisms as a defense against such multiple stressors. We studied, for the first time, expression of stress proteins, heat shock proteins (HSP) mitogen-activated kinase (MAPK), Daphnia magna natural population to environmental changes heavily modified water body (Lake Koronia, Northern Greece). In paralle...

High temperature has become a global concern because it seriously affects the growth and reproduction of plants. Exposure of plant cells to high temperatures result in cellular damage and can even lead to cell death. Part of the damage can be ascribed to the action of reactive oxygen species (ROS), which accumulate during abiotic stresses such as heat stress. ROS are toxic and can modify other ...

Exposure of NIH3T3 cells to elevated temperatures induces the phosphorylation and activation of mitogen-activated protein (MAP) kinases [or extracellular signal-regulated kinases (ERKs)]. To investigate the significance of MAP kinase activation by heat shock, we examined the effect of inhibiting the activity of MAP kinase on heat shock protein 70 (hsp 70) expression. Overexpression of a dominan...

Theileria equi and Babesia caballi are the causative agents of equine piroplasmosis (EP). Currently, imidocarb dipropionate (ID) is only available drug for treating clinical form EP. Serious side effects incomplete clearance infection a major drawback ID. Heat-shock proteins (Hsp) play vital role in life cycle these haemoprotozoans by preventing alteration protein conformation. These Hsp activa...

In the present study, we compared the induction of heat shock proteins (HSPs) by heat and heavy metal ions in three different endothelial cell types, namely, human umbilical vein endothelial cells, human pulmonary microvascular endothelial cells, and the cell line EA.hy 926. Our results show that especially Zn2+ and Cd2+ are inducers of 70-kDa (HSP70), 60-kDa (HSP60), 32-kDa (HSP32), and 27-kDa...

SYNOPSIS. This paper reviews the generalized stress response in fish at the cellular and neuroendocrine levels. The focus of this review is to examine the possible relationships between the stress responses at these two levels in fish. It focuses primarily on the heat shock protein 70 (hsp70). Thus, the descriptions of the endocrine and the cellular stress responses are followed by a discussion...

The characteristic heat shock or stress response is induced upon exposure of an organism (cell) to a number of environmental stressors, e.g. heat, anoxia and heavy metals (reviewed in Morimoto et al. 1990; Ellis and van der Vies, 1991). The heat shock or stress proteins are the most broadly distributed class of proteins known and amongst the most highly conserved. These stress proteins and thei...

CD4+ T cells are subdivided into Th1 and Th2 cells. Their relative presence or activation is thought to have a regulatory effect on immune behaviour. Until recently, the relative suppression of Th1 cells by the relative increase of Th2 activities, was thought to be a main mechanism of keeping or restoring the balance in a diseased immune system. Nowadays, however, a specialised subset of regula...

The chromatin structure of heat shock protein (HSP)-encoding genes undergoes dramatic alterations upon transcriptional induction, including, in extreme cases, domain-wide nucleosome disassembly. Here, we use a combination of gene knock-out, in situ mutagenesis, chromatin immunoprecipitation, and expression assays to investigate the role of histone modification complexes in regulating heat shock...