Abstract Breast and cervical cancers are the leading cause of death in women, chemotherapy with cytotoxins is usual treatment. This study evaluated cytotoxicity guava leaf ( Psidium guajava L.) extracts as an alternative chemotherapeutic drug. Although many studies related to cytotoxic effects on cancer cells have been reported, fractions human breast (T47D, MCF-7, HeLa) never evaluated. Herein...

poliomyelitis, an acute viral infectious disease caused by poliovirus, still remains a public health problem in developing countries. despite the global effort to eradicate polio, continuing the polio immunization with a potent and safe vaccine is essential. for accurate vaccine evaluation, three types of cell lines including hela, hep2c and vero were evaluated and compared using two methods of...

Curcuma purpurascens Bl., belonging to the Zingiberaceae family, is known as temu tis in Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-M...

The present study investigated the effects of valerian methanolic extract and valerenic acid on expression LL-37 gene protein in A549 MRC5 line cells. After preparing Valerian seeds, sowing them March 2020, harvesting rhizome October was prepared from by maceration method. content determined using high performance liquid chromatography (HPLC). Two cell lines (A549 MRC-5) were used to extract, M...

Low power femtosecond-pulsed NIR irradiation kills HeLa cells incubated with Ag nanoplates by both photothermal effect and optical breakdown leading to plasma formation that spreads fast through the cell.

PURPOSE The interference of electric fields (EF) with biological processes is an issue of considerable interest. No studies have as yet been reported on the combined effect of EF plus ionising radiation. Here we report studies on this combined effect using the prokaryote Microcystis panniformis, the eukaryote Candida albicans and human cells. MATERIALS AND METHODS Cultures of Microcystis pann...

We evaluated the in vitro antiproliferative and apoptotic potential of the ethyl acetate extract (EAE) of Peltophorum africanum, a member of the family Fabaceae (Sond) in order to validate its pharmacological use. Antiproliferation of human breast (MCF-7), colon (HT-29) and cervical (HeLa) cancer cell lines by EAE was investigated using the Cell Titer-Blue viability assay and the mechanism of a...

sulfonamides are the first effective chemotherapeutic agents used for several years to cure or prevent systemic bacterial infections. in addition, this agents showed anti-carbonic anhydrase and cause cell cycle perturbation in the g1 phase, disruption of microtubule assembly, suppression of the transcription activator nf-y, angiogenesis and matrix metalloproteinase (mmp). in recent years, novel...

Human parainfluenza type 2 virus (hPIV-2)-infected HeLa (HeLa-CA) cells and hPIV-2 V-expressing HeLa (HeLa-V) cells show high resistance to alpha/beta interferons (IFN-alpha/beta) irrespective of whether vesicular stomatitis virus or Sindbis virus is used as a challenge virus. When Sindbis virus is used, these cells show high susceptibility to human IFN-gamma. Furthermore, the multiplication of...