objective: herpes simplex virus (hsvs) is a widespread human infectious agent, responsible for persistent and latent infections. herpes simplex virus infections are usually continually recurrent in the normal population and represent a significant cause of complications in immunocompromised patients. materials and methods: in this study hsvs were propagated in bk cells and more than 502 sample...

this study was conducted to evaluate the effects of administering oral acyclovir in conjunction with topical acyclovir and steroids in the treatment of necrotizing herpetic keratouveitis. all patients with necrotizing herpetic keratouveits who have been consulted during 1996-1997 at the farbi eye hospital were studied . the patients were randomly assigned to two groups. in the first group, topi...

Herpes simplex virus is responsible for numerous ocular diseases, the most common of which is herpetic stromal keratitis. This is a recurrent infection of the cornea that typically begins with a subclinical infection of the cornea that establishes a latent infection of sensory ganglia, most often the trigeminal ganglia. Recurring infections occur when the virus is reactivated from latency and t...

The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for...

acute idiopathic peripheral facial paralysis (bell's palsy) is the most common disorder of the facial nerve. most patients recover completely, although some have permanent disfiguring facial weakness. many studies have attempted to identify an infectious etiology for this disease. although the cause of bell's palsy remains unknown, recent studies suggest a possible association with herpes simpl...

We describe the mapping and sequencing of mutations within the DNA polymerase gene of herpes simplex virus type 1 which confer resistance to aphidicolin, a DNA polymerase inhibitor. The mutations occur near two regions which are highly conserved among DNA polymerases related to the herpes simplex enzyme. They also occur near other herpes simplex mutations which affect the interactions between t...

AIM To study herpes simplex virus (HSV) DNA in tears from patients with atypical epithelial keratitis of unknown aetiology. METHODS Tear samples were collected from 17 affected eyes of 17 consecutive patients suffering from epithelial keratitis in whom HSV keratitis was suspected but whose diagnosis was difficult on the basis of clinical manifestations alone. Using reduced sensitivity polymer...

PURPOSE To comparatively analyze the methodological efficacy of the polymerase chain reaction (PCR) assay for herpes simplex virus 1 (HSV) detection in tears. METHODS This retrospective study reviewed the medical records of 115 patients who were clinically diagnosed with herpes keratitis, and their tear samples were collected for HSV detection. PCR positive rates were analyzed for their depen...

PURPOSE The antiviral activity of Indian Medicinal plant extract Swertia chirata was tested against Herpes simplex virus (HSV) type-1, using multiple approaches both at cellular and molecular level. METHODS Cytotoxicity, plaque reduction, virus infectivity, antigen expression and polymerase chain reaction (PCR) assays were conducted to test the antiviral activity of the plant extract. RESUL...

results among the 181 anti-hcv ab negative patients, 13 (7.2%) were positive for hcv core ag and 11 (6%) had detectable hcv rna with a range of 40-336543 iu/ml by pcr. the two tests had a high measurement agreement (kappa=0.82, p<0.001). of the 13 patients with positive hcv core ag test results, 3 were negative for hcv rna. considering real time pcr for hcv rna as the gold standard for hcv infe...