Chromatographic procedures for the separation and identification of actinomycins have been reviewed1}. Only one report of HPLC has appeared2); this described reversed-phase separations of the C complex and of a biosynthetic mixture of actinomycin D (AMD)and congeners containing c/s-4-chloroproline in place of one or both prolines. In the present study a wider range of naturally occurring actino...

An improved high-performance liquid-chromatographic (HPLC) assay for the simultaneous determination of serotonin (5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) in human urine is reported. Following the automated precolumn derivatization of urinary 5-HT and 5-HIAA with benzylamine, the derivatives are separated by isocratic elution on a reversed-phase C18 semi-microbore column, and are fluoro...

The measurement of glycosylated haemoglobin in blood is vital for monitoring metabolic control in diabetics [1-3]. The development of many different methods of measurement (based on cation-exchange chromatography, electrophoresis, high performance liquid chromatography [HPLC], affinity chromatography, radio and colorimetric immuno techniques) proves the importance of this assay [4]. Since the f...

N,N'-Methylenebisacrylamide (MBAA), which is an important raw material in a widely industrial area, was synthesized using convenient catalysts. The synthesized monomer was fully characterized by the elemental analysis, fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), 1H-NMR, 13C-NMR, GC/MS, and high performance liquid chromatography (HPLC) analysis. Six differe...

An analytical method based on high-performance liquid chromatography-photodiode array detection was developed for the simultaneous determination of three anti-proliferative withanolides [withalongolide A ( 1: ), withaferin A ( 2: ) and withalongolide B ( 3: )] present in the aboveground biomass of the long-leaf groundcherry, Physalis longifolia. This method was achieved by biomass extraction fo...

A protected S-acetylthio porphyrin was synthesized and attached to the Au38(2-phenylethanethiolate)24 cluster in a ligand exchange reaction. Chiral high performance liquid chromatography of the functionalized cluster yielded enantiomeric pairs of clusters probably differing in the binding site of the porphyrin. As proven by circular dichroism, the chirality was maintained. Exciton coupling betw...

The publisher regrets that the following correction was not implemented in the final proof of this contribution. It should read throughout the entire article " ultra high performance liquid chromatography ". Our sincere apologies to the authors.

BACKGROUND Determining folate intake is difficult because existing folate data in food-composition tables are scarce and unreliable. OBJECTIVE The purposes of this study were first to analyze 125 of the most important foods that contribute to folate intake in the Netherlands and second to estimate the folate intake of a representative sample of the population. DESIGN We analyzed the folate ...

An high-performance liquid chromatography (HPLC) method for identification of quaternary and non-quaternary compounds (parent compounds, intermediates, by-products, and products) within the synthesis of the acetylcholinesterase reactivator HI-6, the most promising antidote of nerve agent poisonings, is described. This HPLC method could be of high interest as a quick purity control for those who...

We employ reversed-phase ion-pair high-performance liquid chromatography to quantitatively characterize the oxidation kinetics of thiourea oxidation by hydrogen peroxide. The HPLC technique makes it possible to monitor the concentrations of a variety of sulfur-containing species with different oxidation states and to elucidate the relative phase relations among them. The experimental results ar...